BMP-2在减阻牵张快速牙移动不同加力方式下的表达及对牙移动的影响  

The effects of BMP-2 expression in the related periodontal tissue on bone remolding of rapid tooth movement by resistance reduction and distration method

作　　者：彭早霞[1] 李宁 李佩[3] 李美静[3] 杨乐乐[3] 陈曦[3] 米丛波[1] 

机构地区：[1]新疆医科大学第一附属医院口腔正畸科,乌鲁木齐830054 [2]烟台市口腔医院正畸科 [3]西安交通大学第一附属医院口腔科

出　　处：《实用口腔医学杂志》2016年第1期53-57,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81070863);陕西省科技攻关项目(编号:2014K11-03-08-02)

摘　　要：目的:观察不同牵张力下减阻牵张快速牙移动中BMP-2的表达。方法:Beagle犬12只,随机均分为加力5、15 d、加力15 d保持固定10、90 d 4组。以■为移动牙每个组3只犬的6颗移动牙随机采用减阻—牵张方法、减阻-常规方法和常规方法各2颗。各组犬按预定时间处死并获取移动牙牙周组织块,免疫组化法染色并观察BMP-2表达。结果:各加力方式下BMP-2阳性表达分布区域相似,均在加力结束时达峰值,其中减阻牵张组峰值最大(P<0.05);牙移动距离最大(P<0.01)。加力各时间点,减阻常规组BMP-2阳性表达均强于常规方法组但不及减阻牵张组显著;保持固定90 d,3组无差异(P>0.05)。结论:减阻措施配合持续强牵张力可显著提高移动牙牵张新骨区BMP-2阳性表达加速牙周组织新骨形成。Objective： To investigate the effects of bone morphogenetic protein 2 （BMP-2） expression in the related periodontal tissue on bone remodeling under different distracting force during rapid tooth movement by resistance reduction and distraction. Methods： 12 Beagle dogs were randomly divided into 4 groups as follows： 5 d distraction, 15 d distraction, 15 d distraction and 10 d retaining and 15 d distraction and 90 d retaining. 4┯4 were distalized. 6 teeth in each group were randomly assigned to re- sistance and distracting method, resistance and conventional method and conventional method, and there were 2 teeth in each group. Moving teeth models were prepared regularly. BMP-2 expression was examined by immunohistochemistry. Results： The BMP-2 positive expression of the 3 groups of different distraction schedule showed similar distribution area, and it reached peak at the end of 15-day distration, but the group of resistance and distracting method showed the maximum peak （P 〈 0.05 ） and maximum tooth movement（ P 〈 0. O1 ）. In different stages of distraction, the positive expression in the group of resistance reduc- tion and conventional method was higher than in the group of conventional method but lower than the group of resistance reduction and extraction method（ P 〈0.05 ）. There was no significant difference in BMP-2 positive expression among 3 groups when retai- ning was 90 d（ P 〉 0.05 ）. Conclusion： Resistance reduction with sustained strong distracting force can significantly increased the positive expression of BMP-2 and effectively accelerate new bone formation in periodontal tissue.

关 键 词：减阻牵张 快速牙齿移动 BMP-2 骨改建 

分 类 号：R783.5[医药卫生—口腔医学]