微沟槽形貌对人牙龈成纤维细胞黏附及细胞周期推进影响的研究  

The effects of microgroove surface form of titanium on the adhesion and cell cycle progression of human gingival fibroblasts

作　　者：赖颖真[1] 林珊[2] 陈江[3] 

机构地区：[1]厦门医学高等专科学校口腔医学系,361000 [2]福建医科大学附属第一医院口腔科 [3]福建医科大学附属口腔医院种植科

出　　处：《实用口腔医学杂志》2016年第1期89-95,共7页Journal of Practical Stomatology

摘　　要：目的:研究钛微沟槽形貌对人牙龈成纤维细胞黏附及细胞周期推进的影响。方法:用光刻技术在硅片表面制作钛微沟槽形貌,沟槽宽度(等于间隔)分别为15、30、60μm,沟槽深度为5μm和10μm,分组为T15/5、T15/10、T30/5、T30/10、T60/5、T60/10,光滑钛(T0)表面为对照组,测量材料表面微沟槽形貌,将体外培养的人牙龈成纤维细胞(HGFs)接种于材料表面,用免疫荧光和流式细胞仪周期分析观察细胞黏附形态及细胞周期推进的差异。结果:T0组表面细胞排列不规则,沟槽表面细胞均顺沟槽排列,T60/10组细胞周期S期比例始终高于其他各组,T30组居中,T15组最小,但仍高于对照T0组,宽度大的沟槽组(T60,T30)沟槽深度的增加促进细胞周期S期比例的增加,而沟槽宽度小的T15组,沟槽加深减少S期比例。结论:本实验所设计的沟槽尺寸均能诱导细胞顺着沟槽排列,随着沟槽的宽度增加、深度增大越有利于促进细胞周期的推进。Objective： To investigate the effect of microgroove surfaces of titanium on the adhesion and cell cycle progression of human gingival fibroblasts（HGFs）. Methods： Microgroove titanium surfaces were fabricated on silicon plate by photolithography with parallel grooves： 15, 30 and 60μm in width and 5 μm and 10 μm in depth, the groups were denoted as T15/5, T15/10, T30/5, T30/10, T60/5 and T60/10, respectively. Smooth titanium surfaces （T0） were used as the controls. Surface topography were observed by ES- EM. HGFs were cultured on the microgroove surfaces. Morphology of ＂contact guidance＂ was observed by immunofluorescence tech- nique. Cell cycle distribution was analyzed by Flow cytometry. Results： HGFs on the microgroove surfaces had ＂contact guidance＂ par- allel to the microgrooves, whereas the cells on TO were oriented randomly. T60/10 group had the highest percentage of S phase cells, followed by T30 group and T15 group, but still higher than that in the control group. In groups with higher groove width （ T60 group and T30 group）, the increase of groove depth benefited the increase of S phase percentage, while in T15 group, the increase of groove width decreased the S phase percentage. Conclusion： Surfaces of microgrooves with different dimensions achieved ＂contact guidance＂ for the cultured HGFs. The surfaces with increasing groove width and depth benefit the cell cycle progression.

关 键 词：软组织 种植体 微沟槽 人牙龈成纤维细胞 接触诱导 细胞周期 

分 类 号：R783[医药卫生—口腔医学]