早期腹腔置管引流对大鼠重症急性胰腺炎相关肠黏膜损伤的作用  

Effect of early peritoneal drainage on the injury of intestinal mucosa in the rats with severe acute pancreatitis

作　　者：邓超[1] 曹均强 梁鸿寅[1] 陈光宇[1] 刘卫辉[1] 汤礼军[1] 戴睿武[1] 

机构地区：[1]成都军区总医院全军普外中心,成都610083

出　　处：《解放军医学杂志》2016年第2期119-122,共4页Medical Journal of Chinese People's Liberation Army

基　　金：四川省科技厅应用基础研究课题(2013JY0046)~~

摘　　要：目的研究早期腹腔置管引流对大鼠重症急性胰腺炎(SAP)相关肠黏膜损伤的保护作用。方法 36只SD大鼠随机分为对照组、模型组(SAP组)、早期腹腔置管引流组(APD组),每组12只。向胰胆管以12ml/h的速度注入5%牛磺胆酸钠(0.1ml/100g),制作大鼠SAP模型。造模后24h处死,观察并对比各组大鼠腹腔内大体改变,HE染色观察小肠病理改变并评分,ELISA法测定大鼠血清中肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)、二胺氧化酶(DAO)及D-乳酸水平。结果与对照组比较,SAP组、APD组小肠组织病理学评分,血清TNF-α、IL-1β、二胺氧化酶及D-乳酸水平均显著升高(P<0.05);与SAP组比较,APD组上述各项指标均显著降低(P<0.05)。结论早期腹腔置管引流能有效改善SAP大鼠模型肠黏膜损伤程度,减轻机体全身炎症反应。Objective To study the protective effect of early peritoneal drainage against injury of intestinal mucosa in rats with severe acute pancreatitis. Methods Thirty-six SD rats were randomly and equally divided into three group, i.e. control group, model（SAP） group, drainage（APD） group. Severe acute pancreatitis was induced in rats by injecting 5% sodium taurocholate（2ml/h, 0.1ml/100g） into the common biliopancreatic duct. All the rats were sacrificed 24 hours after operation. The condition of abdominal cavity was observed, and pathological changes in ileum were detected by HE staining. The serum levels of TNF-α, IL-1β, diamine oxidase（DAO） and D-lactate were determined by ELISA. Results Compared with the control group, the histopathological changes in ileum were more marked, and serum levels of TNF-α, IL-1β, DAO and D-lactate were significantly higher in SAP group and APD group（P0.05）; Compared with SAP group, the histopathological changes in ileum were less marked, and serum levels of TNF-α, IL-1β, DAO and D-lactate were significantly lower in APD group（P0.05）. Conclusion Early peritoneal drainage can effectively improve the intestinal mucosal injury in acute pancreatitis rats, and reduce the systemic inflammatory response.

关 键 词：引流术 胰腺炎 急性坏死性 肠黏膜 

分 类 号：R576.1[医药卫生—消化系统]