Id1基因对人骨肉瘤细胞恶性逆转向成骨分化的影响  

Effect of Id1 gene on reversing malignant biological behaviors and inducing osteogenic differentiation of human osteosarcoma cells

作　　者：仇超[1] 康权[1] 迭小红[1] 罗光金 吕凤香[1] 肖程[1] 罗庆[1] 

机构地区：[1]重庆医科大学附属儿童医院儿科研究所儿童肿瘤研究室,儿童发育疾病研究教育部重点实验室,儿科学重庆市重点实验室,重庆市儿童发育重大疾病诊治与预防国际科技合作基地,重庆400014

出　　处：《第三军医大学学报》2016年第4期344-349,共6页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(81172545);重庆市自然科学基金一般项目(CSTC2012jj A10120;CSTC2013jcyj10026)~~

摘　　要：目的探讨分化抑制因子1(inhibitor of differentiation factor 1,Id1)对人骨肉瘤细胞MG63恶性逆转向成骨分化的影响。方法用重组腺病毒调控MG63细胞中Id1过表达后,RT-PCR及Western blot检测验证Id1的表达情况,CCK-8检测细胞增殖能力,划痕实验检测细胞迁移能力,Transwell实验检测细胞侵袭能力,流式细胞术检测细胞周期,ALP读数法检测成骨分化早期指标ALP的活力。结果 Ad Id1重组腺病毒可以使MG63细胞中的Id1表达量增高,Adsi Id1重组腺病毒可以使MG63细胞中的Id1表达量降低(P<0.05);Id1过表达后,MG63细胞的增殖能力、迁移能力、侵袭能力等恶性生物学行为均增强,G1期细胞比例降低,Id1表达量下调后则逆转MG63细胞的恶性生物学行为,G1期细胞比例增高(P<0.05);Id1表达量下调后,正常成骨分化的早期指标碱性磷酸酶(ALP)表达量增高(P<0.05)。结论抑制Id1基因表达可以促使骨肉瘤的恶性生物学行为逆转并诱导骨肉瘤细胞发生成骨分化。Objective To investigate the effect of inhibitor of differentiation factor 1 （ Idl ） on reversing the malignant biological behaviors and inducing osteogenic differentiation of human osteosarcoma cells. Methods Human osteosarcoma cell line MG63 was infected with recombinant adenoviruses AdIdl, AdsiIdl and AdGFP separately. RT-PCR and Western blot assay were used to determine the expression of Idl in MG63 cells, and the cell proliferation, migration and invasion were detected by CCK-8 assay, scratch wound assay, and Transwell assay, respectively. Alkaline phosphatase （ALP） numeration was used to detect the early osteogenic potential of MG63 cells, and the cell cycle was detected by flow cytometry. Results The mRNA and protein expression of Idl gene was increased after infection with AdIdl and decreased after infection with AdsiIdl （ P 〈 0. 05 ）. CCK-8 assay, scratch wound assay and Transwell assay results showed that the malignant behaviors including cell proliferation, migration and invasion were significantly increased after AdIdl infection, and significantly decreased after AdsiIdl infection （P 〈 0. 05 ）. The percentage of Gl- phase cells was decreased after infection with AdIdl and increased after infection with AdsiIdl （P 〈 0.05 ）. AdsiIdl infection could enhance the activity of ALP in MG63 cells （P 〈 0. 05）. Conclusion Inhibiting the expression of Idl gene can reverse the malignant biological behaviors and induce the osteogenic differentiation of human osteosarcoma cells.

关 键 词：骨肉瘤 分化抑制因子 增殖 迁移 侵袭 分化 

分 类 号：R73-362[医药卫生—肿瘤] R730.23[医药卫生—临床医学]