高效液相-串联质谱法同时检测鸡蛋中金刚烷胺与四种氟喹诺酮类药物残留的研究  

Simultaneous Determination of Amantadine and four Kinds of Quinolones in Eggs by High Performance Liquid Chromatography with Tandem Mass Spectrometry

作　　者：王威利[1,2] 吴维煇[1] 何绮霞[1] 殷秋妙[1] 续倩[1] 张展[1,2] 林雪贤 

机构地区：[1]广东省农业科学院农产品公共监测中心,广东广州510640 [2]农业部农产品质量安全风险评估实验室(广州),广东广州510640 [3]农业部农产品质量安全检测与评价重点实验室,广东广州510640

出　　处：《中国家禽》2016年第2期29-32,共4页China Poultry

基　　金：国家农产品质量安全风险评估项目(GJFP201500801;GJFP201500802)

摘　　要：试验建立了可同时测定鸡蛋中金刚烷胺、环丙沙星、恩诺沙星、达氟沙星和沙拉沙星残留量的高效液相-串联质谱法（HPLC-MS/MS）。样品经1%甲酸乙腈提取、氮吹浓缩、复溶、正己烷脱脂净化等步骤处理后,用HPLC-MS/MS检测。结果表明：5种药物在1~50μg/L浓度范围内具有良好的线性关系,空白样品同时添加5种药物的回收率均在76.6%~102.1%之间;定量限为1μg/kg,检出限为0.5μg/kg。该方法操作简单、快速、灵敏、高效。A method of high performance liquid chromatography with tandem mass spectrometry （HPLC-MS/MS） for simultaneous determination of amantadine, ciprofloxacin, enrofloxacin, danofloxacin, sarafloxacin in eggs was developed. The samples were extracted by 1% formic acid in acetonitrile,then the extract was condensed under nitrogen,dissolved in 50% acetonitrile aqueous solution （v/v）,the samples were analyzed by HPLC-MS/MS after purified using n-hexane for defatting. The results showed that five compounds had good linearity at a concentration range of 1 to 50 μg/L.The average recoveries of five drugs were between 76.6% to 102.1%. The limit of quantitation （LOQ） was 1 μg/kg and the limits of detection （LOD） was 0.5 μg/kg. This method was simple,fast,sensitive and efficient.

关 键 词：鸡蛋 HPLC-MS/MS 金刚烷胺 氟喹诺酮类药物 

分 类 号：S859[农业科学—临床兽医学] O657[农业科学—兽医学]