IL-24抑癌基因联合大蒜素对肺癌细胞的杀伤作用  

The inhibition of lung cancer by IL-24 tumor suppressor combined with allicin in vitro

作　　者：黄子轩[1] 李红[2] 代龙光 张雪[1] 林敏[1] 李正祎[3] 

机构地区：[1]吉林医药学院,吉林吉林132013 [2]吉化总医院一院急诊科,吉林吉林132021 [3]吉林医药学院检验学院,吉林吉林132013

出　　处：《吉林医药学院学报》2016年第1期15-19,共5页Journal of Jilin Medical University

基　　金：吉林省大学生创新创业训练计划项目

摘　　要：目的探讨IL-24基因联合大蒜素对肺癌A549细胞的增殖抑制和促凋亡作用。方法提取前期构建的p DC316-h IL-24-EGFP质粒,转染肺癌A549细胞,分为A组:空白对照组;B组:单独大蒜素组;C组:脂质体+IL-24组;D组:大蒜素+脂质体+IL-24。荧光显微镜下观察质粒的转染情况,结晶紫染色法和MTT法检测p DC316-h IL-24-EGFP联合大蒜素对肺癌A549细胞生长的影响,用流式细胞仪检测细胞凋亡。结果 40、80 mg/L大蒜素和p DC316-h IL-24-EGFP单用24、48 h对A549细胞均有明显抑制作用,呈剂量依赖性。低剂量大蒜素(40 mg/L)联合p DC316-h IL-24-EGFP比单用p DC316-h IL-24-EGFP作用更强(P<0.05)。40 mg/L大蒜素、p DC316-h IL-24-EGFP和两药联用48 h,A549细胞凋亡率分别为32.7%,39.4%和68.8%。结论 p DC316-h IL-24-EGFP联合大蒜素对人肺癌A549细胞有协同抑制作用。Objective To investigate the inhibitory effect of combined treatment of IL-24 gene with allicin on A549 lung cancer cell lines. Methods Pre-constructed recombinant plasmid pDC316-hIL-24-EGFP was extracted and tranfected to A549 cells. There were four groups：Group A（ blank control） ,Group B（ allicin） ,Group C（ pDC316-hIL-24-EGFP with lipofectamine transfection）,Group D（pDC316-hIL-24-EGFP with lipofectamine transfection combined with allicin） . The transduction efficiency of target cells was detected by fluorescent microscope. Cytotoxic effects of pDC316-hIL-24-EGFP combined with allicin on A549 was detected by crystal violet staining and MTT assay. Results Either allcin （40 mg/L or 80 mg/L） or pDC316-hIL-24-EGFP could inhibit the growth of A549 lung cancer cells in a dose dependent manner. Combined treatment of allcin 40 mg/L with pDC316-hIL-24-EGFP resulted in asynergistic effect of inhibiting cell growth（P〈0. 05）. After incubation with allcin 40 mg/L,pDC316-hIL-24-EGFP and in combination,the apoptosis rate of A549 cells was 32. 7%,39. 4% and 68. 8%,respectively. Conclusion The combination of pDC316-hIL-24-EGFP and allcin has a synergistic effect on the growth of A549 cells.

关 键 词：IL-24基因 大蒜素 联合用药 人肺腺癌A549细胞 

分 类 号：R285[医药卫生—中药学]