Characterization and identification of enzyme-producing microflora isolated from the gut of sea cucumberApostichopus japonicus  

作　　者：李凤辉 高菲 谭杰 范超晶 孙慧玲 燕敬平 陈四清 王肖君 

机构地区：[1]Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China [2]College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China [3]Zhaoyuan Marine Environmental Monitoring Station, Yantai 265400, China

出　　处：《Chinese Journal of Oceanology and Limnology》2016年第1期153-162,共10页中国海洋湖沼学报（英文版）

基　　金：Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A412);the National Natural Science Foundation of China(No.41106145);the National Key Technology R&D Program of China(No.2011BAD13B02);the Science and Technology Development Planning Project of Shandong Province(No.2012GGA06021);the Key Laboratory of Mariculture&Stock Enhancement in North China’s Sea,Ministry of Agriculture,China(No.2014-MSENC-KF-03)

摘　　要：Gut microorganisms play an important role in the digestion of their host animals. The purpose of this research was to isolate and assess the enzyme-producing microbes from the Apostichopusjaponicus gut. Thirty-nine strains that can produce at least one of the three digestive enzymes （protease, amylase, and cellulase） were qualitatively screened based on their extracellular enzyme-producing abilities. The enzyme-producing strains clustered into eight groups at the genetic similarity level of 100% by analyzing the restriction patterns of 16S rDNA amplified with Mbo L Phylogenetic analysis revealed that 37 strains belonged to the genus Bacillus and two were members of the genus Virgibacillus. Enzyme-producing capability results indicate that the main enzyme-producing microflora in the A.japonicus gut was Bacillus, which can produce protease, amylase, and cellulase. Virgibacillus, however, can only produce protease. The high enzyme-producing capability of the isolates suggests that the gut microbiota play an important role in the sea cucumber digestive process.Gut microorganisms play an important role in the digestion of their host animals. The purpose of this research was to isolate and assess the enzyme-producing microbes from the Apostichopus japonicus gut. Thirty-nine strains that can produce at least one of the three digestive enzymes(protease,amylase,and cellulase) were qualitatively screened based on their extracellular enzyme-producing abilities. The enzyme-producing strains clustered into eight groups at the genetic similarity level of 100% by analyzing the restriction patterns of 16 S rDNA amplified with Mbo I. Phylogenetic analysis revealed that 37 strains belonged to the genus Bacillus and two were members of the genus Virgibacillus. Enzyme-producing capability results indicate that the main enzyme-producing microflora in the A. japonicus gut was Bacillus,which can produce protease,amylase,and cellulase. Virgibacillus,however,can only produce protease. The high enzyme-producing capability of the isolates suggests that the gut microbiota play an important role in the sea cucumber digestive process.

关 键 词：Apostichopusjaponicus GUT enzyme-producing microorganisms 16S rDNA-RFLP aquaculture probiotics 

分 类 号：S917.4[农业科学—水产科学]