石墨烯/纳米纤维素/亚甲基蓝电极同时检测抗坏血酸和尿酸  

Simultaneous determination of ascorbic acid and uric acid at GN/NCC/MB/GCE modified electrode

作　　者：窦砚鹏 梁英[1] 彭俊军[2] 刘慧宏[2] 

机构地区：[1]湖北文理学院化学工程与食品科学学院,湖北襄阳441053 [2]武汉纺织大学化学与化工学院,湖北武汉430074

出　　处：《湖北大学学报（自然科学版）》2016年第1期73-78,共6页Journal of Hubei University：Natural Science

基　　金：国家自然科学基金(51378183)资助

摘　　要：直接采用电化学还原法在玻碳电极上成功制备石墨烯（graphene,GN）修饰的电极和石墨烯/纳米纤维素/亚甲基蓝（graphene/nano-crystalline cellulose/methylene blue composite,GN/NCC/MB）复合材料修饰电极,并利用循环伏安法研究了这两种电极在PBS（phosphate buffered solution 0.1 mol·L-1,pH=7.0）溶液中的对抗坏血酸（ascorbic acid,AA）和尿酸（uric acid,UA）在该电极上的电化学行为.实验结果表明：在AA和UA共存体系中,GN/NCC/MB/GCE修饰的玻碳电极能够将AA和UA的氧化峰电位明显分开,峰电位相差300mV.并且用两种形式,研究了AA和UA两种物质峰电流与其物质的量浓度的关系,发现AA、UA的氧化峰电流与其浓度分别在40.0~700.0μmol·L-1、4.0~120.0μmol·L-1范围内呈线性关系.在AA和UA共存时,两者的最低检测限度分别为17.644 2μmol·L-1和3.251 1μmol·L-1.GN/NCC/MB复合材料修饰的玻碳电极可以实现AA和UA共存时两种物质的定量检测.GN modified electrode and GN/NCC/MB composite modified electrode were prepared by direct electrochemical reduction method,and was used to study the electrochemical behavior of ascorbic acid and uric acid by cyclic voltammetry in PBS solution（0.1mol·L-1,pH=7.0）.The experiments results show that the oxidation peaks potential of AA and UA can be distinguished to 0Vand 0.3Vat a scan rate of 20mV·s-1 respectively,with a potential difference of 300 mV,which is large enough to determine UA and AA individually and simultaneously.In the mixed solution of AA and UA,two experimental modes were adopted to study the linear relationship between the oxidation peak current of AA（UA）and the concentration of AA（UA）,the experiments shows that the catalytic peak current obtained is linearly dependent on the UA and AA concentration in the range of 40.0—700.0μmol·L-1,4.0—120.0μmol·L-1 in 0.1mol·L-1（pH=7.0）,and the detection limit is 17.644 2μmol·L-1 and 3.251 1μmol·L-1,respectively.GN/NCC/MB composite modified electrode can realize the quantitative determination of AA and UA in the mixed solution simultaneously.

关 键 词：石墨烯 纳米纤维素 亚甲基蓝 抗坏血酸 尿酸 修饰电极 

分 类 号：TQ32[化学工程—合成树脂塑料工业]