多孔微球种子细胞储库型组织工程肌腱支架体外构建  

Porosity Microsphere as Seed Cells Storage to Build Tissue Engineering Tendon Scaffold in Vitro

作　　者：梁文清[1] 周国芳[1] 钱宇[1] 徐国健[1] 谢明华[1] 

机构地区：[1]浙江省绍兴市人民医院骨科,浙江绍兴312000

出　　处：《中国中医骨伤科杂志》2016年第1期9-12,17,共5页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：浙江省自然科学基金资助项目(LY13H060005);浙江省公益性技术应用研究计划项目(2014C33254);浙江省医药卫生省部共建培育计划项目(2015PYA012);绍兴市公益性应用计划项目(2013B70081)

摘　　要：目的:以多孔微球作为种子细胞储库,构建体外组织工程肌腱支架。方法:分离、培养大鼠骨髓间充质干细胞(BMSCs)作为种子细胞,复乳法制备多孔微球并接种种子细胞。编织去细胞肌腱支架,并测定其力学特征及孔隙率。将种子细胞微球储库接种在编织去细胞肌腱支架上,测定胶原I,Ⅲ(Collagen I,Ⅲ)的基因表达。结果:BMSCs细胞可以进行成骨和成纤维分化,在多孔微球内可生长增殖。编织去细胞肌腱支架有较好的力学特征及较高的孔隙率。将种子细胞微球储库接种在编织去细胞肌腱支架上后,与对照组相比可以分泌更多的Collagen I,Ⅲ.结论:种子细胞微球储库型编织去细胞肌腱组织工程支架保留了较好的组织学形态及生物学特性。Objective： To choose porosity, microsphere as seed cells storage, and to build tissue engineering tendon scaffold in vitro. Methods： The bone marrow mesenchymal stem cells （BMSCs） of rats were isolate and culture as seed cell. The porosity microsphere was prepared by expanding foam method, and then it was implanted into seed cells. The acellular al- logenic tendon was weaved and its porosity and mechanical strength change were analyzed. The BMSCs storage of porosity microsphere were inoculatein into weaved acellular allogenic tendon, and the gene expression of Collagen Ⅰ, Ⅲ were detected. Results： BMSCs cell could differentiate into osteoblast cells and fibroblast cells, and solidly grow interiorly in porosity microsphere. The acellular allogenic tendon scaffold has the better mechanics property and high porosity. BMSCs can secrete more Collagen Ⅰ, Ⅲ when combined cultured with tissue engineering scaffold. Conclusion： The aeellular allogenic tendon scaffold with porosity microsphere as seed cells storage reserve histology and biomechanics property.

关 键 词：多孔微球 骨髓间充质干细胞 同种异体肌腱 组织工程 

分 类 号：R-33[医药卫生]