高架〇迷宫实验:昆明小鼠状态焦虑动物模型的重测信度研究  

Elevated zero maze as an animal model of state anxiety in Kunming mice: test-retest reliability

作　　者：孙世光[1,2,3] 

机构地区：[1]山东中医药大学第二附属医院药学部,山东济南250001 [2]山东省中西医结合医院药学部,山东济南250001 [3]山东中医药大学药学院,山东济南250355

出　　处：《中国药理学通报》2016年第1期133-137,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金青年基金项目(No 81302887);山东省科技发展计划项目(No 2014GSF119034);山东省中医药科技发展计划项目(No 2015-131);山东省中医药科技发展计划项目(No 2013-103);山东省中医药科技发展计划项目(No 2011-107)

摘　　要：目的探讨高架〇迷宫实验(elevated zero maze,EZM)作为昆明小鼠状态焦虑动物模型的重测信度。方法昆明小鼠(4周龄,♂/♀)进行EZM实验,实验间隔1周进行2次,采用摄像系统记录5 min行为变化,包括两臂区进入时间及进入次数;纳入实验参数有:开臂区进入时间百分率(Otime%)、开臂区进入时间(Otime)、闭臂区进入时间(Ctime)、开臂区进入次数百分率(Oentries%)、开臂区进入次数(Oentries)、闭臂区进入次数(Centries)及两臂区进入总次数(Entries);通过组内相关系数(intraclass correlation coefficient,ICC)和kappa一致性系数(kappa agreement index,κ)对EZM进行重测信度检验,并分析性别、重复测量等因素对EZM行为模式的影响。结果 ICC结果显示,Otime%(♂ICC=0.753,P<0.01;♂+♀ICC=0.535,P<0.05)、Otime(♂ICC=0.753,P<0.01;♂+♀ICC=0.535,P<0.05)、Ctime(♂ICC=0.753,P<0.01;♂+♀ICC=0.535,P<0.05)、Oentries(♂ICC=0.719,P<0.01;♀ICC=0.494,P<0.05;♂+♀ICC=0.583,P<0.01)、Centries(♀ICC=0.658,P<0.01;♀ICC=0.508,P<0.05;♂+♀ICC=0.562,P<0.01)、Entries(♂ICC=0.691,P<0.01;♀ICC=0.502,P<0.05;♂+♀ICC=0.574,P<0.01)初测重测信度相对较好,且有统计学意义;κ结果显示,Otime%(♂κ=0.393,P<0.05)、Ctime(♂κ=0.393,P<0.05)、Oentries(♂κ=0.308,P<0.05;♂+♀κ=0.256,P<0.05)、Centries(♂κ=0.427,P<0.01;♂+♀κ=0.238,P<0.05)、Entries(♂κ=0.469,P<0.01)有统计学意义,但初测重测信度相对较差。结论 Otime%、Entries作为EZM评价状态焦虑动物模型的稳定参数,具有尚可接受的重测信度;尤其应注意♂/♀(强烈推荐♂)对EZM重测信度影响效应相对较大,而重复测量影响效应相对较小。Aim To explore the test-retest reliability of elevated zero maze( EZM),as an animal model of state anxiety in Kunming mice. Methods The behavior of adult,male and female,Kunming mice in EZM was recorded twice,for five minutes,with a one-week inter-trial interval. The following parameters were evaluated: percentage of time on EZM open arms( Otime%),time on EZM open arms( Otime),time on EZM closed arms( Ctime),percentage of entries into EZM open arms( Oentries%),entries into EZM open arms( Oentries),entries into EZM closed arms( Centries),total entries into EZM open and closed arms( Entries). Subsequently,the intraclass correlation coefficient( ICC) and the kappa agreement index( κ)were calculated for these parameters. Results ICC for Otime%( ♂ ICC = 0. 753,P < 0. 01; ♂ + ♀ ICC = 0. 535,P < 0. 05),Otime( ♂ ICC = 0. 753,P < 0. 01; ♂ + ♀ ICC = 0. 535,P <0. 05),Ctime( ♂ ICC = 0. 753,P < 0. 01; ♂ + ♀ ICC =0. 535,P < 0. 05),and Oentries( ♂ ICC = 0. 719,P < 0. 01;♀ ICC = 0. 494,P < 0. 05; ♂ + ♀ ICC = 0. 583,P < 0. 01),Centries( ♀ ICC = 0. 658,P < 0. 01; ♀ ICC = 0. 508,P <0. 05; ♂ + ♀ ICC = 0. 562,P < 0. 01),Entries( ♂ ICC =0. 691,P < 0. 01; ♀ ICC = 0. 502,P < 0. 05; ♂ + ♀ ICC =0. 574,P < 0. 05) showed a good and fair test-retest reliability,respectively. Only κ for Otime%( ♂ κ = 0. 393,P < 0. 05),Ctime( ♂ κ = 0. 393,P < 0. 05),Oentries( ♂ κ = 0. 308,P< 0. 05; ♂ + ♀ κ = 0. 256,P < 0. 05),Centries( ♂ κ =0. 427,P < 0. 01; ♂ + ♀ κ = 0. 238,P < 0. 05),Entries( ♂κ = 0. 469,P < 0. 01) displayed a significant but poor reliability between the test-retest trials. Conclusions The parameters Otime% and Entries for Kunming mice in EZM are stable over time,and indicate an acceptable reliability with a one-week inter-trial interval. Meanwhile,more attention should be paid not to repeated measure effect,but to the sex effect( Male animals recommended to EZM,especially).

关 键 词：高架〇迷宫 状态焦虑 动物模型 重测信度 性别 重复测量 昆明小鼠 

分 类 号：R-332[医药卫生] R363.332