基于免疫磁分离的微流控技术在循环肿瘤细胞富集分离中应用的研究进展  

Research progress on enrichment and isolation of circulating tumor cells using immunomagnetic separation based microfluidic technology

作　　者：李福来[1] 孙平风 黄小林[1] 李凡[1] 许恒毅[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,330047 [2]南昌大学第二附属医院妇产科

出　　处：《中华检验医学杂志》2016年第1期64-67,共4页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目（81201691）;高等学校博士学科点专项科研基金（20123601120005）;江西省青年科学家（井冈之星）培养对象项目（20142BCB23004）;江西省科技计划项目（20151BBG70077）

摘　　要：循环肿瘤细胞（CTCs）存在于多种恶性肿瘤患者的外周血中，CTCs的准确检测有助于肿瘤的早期发现，并可用于指导肿瘤患者个体化治疗、肿瘤化疗药物的快速评估和肿瘤的复发检测。然而，肿瘤患者外周血中CTCs的含量极低，对其检测需要高效、特异的富集分离方法。新兴的微流控技术能对微量液体进行精确操控，结合免疫磁分离技术将其应用于CTCs的分离检测可节省血液样本，具有操作简单、分选速度快、特异性强以及纯度高等优点。Circulating tumor cells （CTCs） exists in peripheral blood Of patients with a variety of malignant tumors, the accurate detection of CTCs helps early detection of cancer, and can be used to guide individualized treatment of cancer patients, rapid assessment of tumor chemotherapy drugs and detection of tumor recurrence. However, the CTCs in peripheral blood of cancer patients were extremely low, their detection requires efficient and specific enrichment and separation methods. The new microfluidic technology can accurately control the microliquid preciously, combined with the immunomagnetic separation technology, has been applied in detecting CTCs, which can save the blood samples of CTCs, has the advantages of simple operation, fast sorting speed, high specificity and high purity.

关 键 词：肿瘤细胞 循环 微流体分析技术 免疫磁化分离 

分 类 号：R730.4[医药卫生—肿瘤]