AAVC-Ⅰ对人口腔鳞癌Tca8113细胞GRP78和Caspase-4基因表达的影响  

Effects of AAVC-Ⅰ on GRP78 and Caspase-4 gene expression in human oral squamous cell carcinoma Tca8113

作　　者：任琳琳[1] 柴琳[2] 

机构地区：[1]皖南医学院病理生理学教研室,安徽芜湖241002 [2]皖南医学院口腔医学院,安徽芜湖241002

出　　处：《皖南医学院学报》2016年第1期12-15,共4页Journal of Wannan Medical College

基　　金：皖南医学院重点科研培育基金项目(WK2015Z08)

摘　　要：目的:研究不同浓度的AAVC-Ⅰ对人口腔鳞癌Tca8113细胞GRP78、Caspase-4基因表达的影响。方法:设置正常对照组和AAVC-Ⅰ实验组,Trizol试剂提取各组总RNA,运用RT-PCR法扩增内参基因β-actin及目的基因GRP78、Caspase-4片段,Chemi Doc XRS凝胶成像系统观察电泳结果并采用Quantity One软件测定目的条带灰度值。结果:正常对照组、实验组均扩增出GRP78、Caspase-4目的条带,且随AAVC-Ⅰ浓度增大GRP78、Caspase-4表达增加。结论:高浓度AAVC-Ⅰ可上调GRP78、Caspase-4基因的表达,因此AAVC-Ⅰ可能诱导人口腔鳞癌Tca8113细胞发生了内质网应激。Objective： To observe the effects anti-tumor component-Ⅰ from Agkistrodon acutus venom（ AAVC-Ⅰ） in diverse dosage on the expression GRP78 and Caspase-4 gene in human oral squamous carcinoma Tca8113 cell lines. Methods： The experiment was performed in normal control group and AAVC-Ⅰ group. Trizol reagent was used to extract total RNA in each group,and RT-PCR was performed to amplify the internal control β-actin gene and fragments of GRP78 and Caspase-4. Chemi Doc XRS gel imaging system was used to observe electrophoresis presentation,and Quantity One software to measure the gray value in the target band. Results： Both GRP78 and Caspase-4 were amplified,and the expression of these two genes was up-regulated with increased dose of AAVC-Ⅰ. Conclusion： High dose of AAVC-Ⅰ can increase the expression of GRP78 and Caspase-4 genes,suggesting that AAVC-Ⅰmay induce endoplasmic reticulum stress in the oral squamous carcinoma Tca8113 cell lines.

关 键 词：AAVC-Ⅰ GRP78 Caspase-4 TCA8113 内质网应激 

分 类 号：R739.8[医药卫生—肿瘤]