IL-17在高危型HPV阳性宫颈癌中的表达  

The expression of IL-17 in the cervical cancer with high-risk human papillomavirus infection

作　　者：薛纪森[1] 陈骋[2] 朱华[1] 章圣辉[3] 吕杰强[2] 胡燕[1] 

机构地区：[1]温州医科大学附属第一医院妇科,浙江温州325015 [2]温州医科大学附属第二医院妇科,浙江温州325027 [3]温州医科大学附属第一医院血液内科,浙江温州325015

出　　处：《温州医科大学学报》2016年第1期24-27,32,共5页Journal of Wenzhou Medical University

基　　金：浙江省卫生厅科研基金资助项目(2011KYA107)

摘　　要：目的:通过检测高危型人乳头瘤病毒(HPV)阳性宫颈癌患者体内IL-17的表达水平,探讨IL-17所介导的特异性免疫反应在高危型HPV感染的宫颈癌发生、发展中可能发挥的作用。方法:选取高危型HPV阳性的宫颈浸润性鳞癌患者(宫颈癌组)28例、宫颈高危型HPV感染者(HPV感染组)25例以及对照组30例。采用ELISA法检测各组患者外周血清及宫颈局部组织匀浆上清液中IL-17的表达水平。结果:1宫颈癌组患者外周血清中IL-17水平较对照组明显升高(P<0.05),与HPV感染组比差异无统计学意义(均P>0.05),HPV感染组与对照组间差异无统计学意义(P>0.05);2各组宫颈局部组织匀浆上清液中IL-17的表达水平均明显高于外周血清表达水平,差异均有统计学意义(均P<0.05);3宫颈局部组织匀浆上清液中IL-17表达水平各组间的差异均有统计学意义(均P<0.05),且宫颈癌组局部组织匀浆上清液中IL-17的表达水平高于HPV感染组,HPV感染组高于对照组,差异均有统计学意义(均P<0.05)。结论:IL-17所介导的免疫反应可能参与并促进了高危型HPV的持续性感染,与宫颈癌的发生、发展进程相关。Objective： To investigate the expression of IL-17 in patients diagnosed as cervical squamous cell carcinoma （SCC） with high-risk human papillomavirus （HPV） infection, with the aim to explore the potential role of specific immune response mediated by IL-17 in the disease progression and carcinogenesis of cervical cancer which is closely associated with high-risk HPV infection. Methods： The study groups included 28 cervical SCC patients with high-risk HPV infection and 25 high-risk HPV infected women with normal cervical cytol- ogy. Thirty patients diagnosed as uterine myoma with normal cervical cytology and negative HPV testing were enrolled as the control group. The concentration of IL-17 in serum and supernatant of cervical tissue homogenate were examined with ELISA method. Results： ①The serum concentrations of IL-17 in patients with cervical SCC were significantly higher than those in the control group （P〈0.05）, but there was no significant difference observed between SCC group vs the high-risk HPV infection group （P〉0.05） and the high-risk HPV infection group vs the control group （P〉0.05）; ②The average concentrations of IL-17 in supernatant of cervical tissue ho- mogenate of all groups were obviously higher than those in serum respectively （P〈0.05）; ③The concentrations of IL-17 in supernatant of cervical tissue homogenate differed significantly among the study groups and the con- trol group （P〈0.05）. The concentrations in the cervical SCC group were higher than those in the high-risk HPV infected group, which were much higher than the control group （P〈0.05）. Conclusion： These results indicate that the immune reaction mediated by IL-17 may participate in and promote the persistent infection of high-risk HPV, which is closely associated with the disease 19roffression and carcinogenesis of cervical cancer cervical cancer.

关 键 词：白细胞介素17 宫颈肿瘤 人乳头瘤病毒 

分 类 号：R737.33[医药卫生—肿瘤]