大鼠心脏骤停后血栓弹力图及单核细胞组织因子表达变化的研究  

The Changes of Thrombelastography and Monocyte Tissue Factor in a Rat Model of Cardiac Arrest

作　　者：陈明迪 王鹏[2,1] 姚蓝[1] 刘远山[1] 周莉莉[2] 方向韶[2,1] 黄子通[2,1] 

机构地区：[1]中山大学心肺脑复苏研究所,510120 [2]中山大学孙逸仙纪念医院急诊科

出　　处：《岭南急诊医学杂志》2015年第6期447-449,共3页Lingnan Journal of Emergency Medicine

基　　金：国家自然科学基金(81501137);广东省自然科学基金(2015A030310042)

摘　　要：目的:研究心跳骤停大鼠恢复自主循环后凝血的变化及其机制。方法:通过致颤法建立大鼠心肺复苏模型,恢复自主循环的雄性SD大鼠18只随机平均分为3组(n=6),假手术组,心肺复苏半小时组,心肺复苏1小时组,分别于相应时间点(复苏后半小时,1小时)采血,行血栓弹力图监测,并分离血液单核细胞,检测单核细胞组织因子mRNA水平的表达变化。结果:与对照组相比复苏后半小时组血栓弹力图R值明显缩短(P<0.05);与对照组相比,复苏后半小时及1小时组单核细胞组织因子表达明显增加(P<0.05)。结论 :TEG快速的监测出心肺复苏后早期凝血系统激活;单核细胞组织因子表达增加是心肺复苏后早期凝血激活的原因之一。Objective ：The aim of this study was to characterize coagulation changes during cardiac arrest and post-resuscitation. Methods ： The Sprague-Dwaley rats were randomly divided into three groups（n =6）, shamoperation group, ROSC 30 min group, ROSC 1h group. We performed to detect TEG, TF-m RNA level of mononuclear leucocytes. The TF-m RNA levels of mononuclear leucocytes were measured by Q-PCR. Results ： TEG R time were prolonged in ROSC 30 min group compared with sham-operation group（P〈0.05）. The TF-m RNA level of mononuclear leucocytes were increased in ROSC 30 min group and ROSC1 h group compared with sham-operation group（P〈0.05）. Conclusion： TEG rapid monitoring of abnormal blood coagulation after cardiopulmonary resuscitation.Increased expression of single nuclear cell tissue factor is one of the causes of abnormal coagulation mechanism in the early stage of cardiopulmonary resuscitation.

关 键 词：心肺复苏 凝血异常 血栓弹力图 组织因子 

分 类 号：R541.78[医药卫生—心血管疾病]