骨髓间充质干细胞移植肝硬化大鼠肝脏超微结构、体视学参数和肝功能的变化  

Bone marrow mesenchymal stem cell transplantation for liver cirrhosis in rats: variation of liver ultrastructure, stereology parameters and liver function indexes

作　　者：丁体龙 马艳春 于莉 马勇 

机构地区：[1]解放军第123E院南京军区肝病中心,安徽省蚌埠市233015

出　　处：《中国组织工程研究》2015年第50期8144-8148,共5页Chinese Journal of Tissue Engineering Research

基　　金：南京军区医学科技创新经费资助项目重点课题(11Z012)~~

摘　　要：背景:间充质干细胞移植治疗肝硬化的疗效在学术界尚有一定的争议,对受体组织结构和功能的影响有待进一步研究。目的:探讨骨髓间充质干细胞对肝硬化大鼠肝脏超微结构、体视学参数和肝功能的影响。方法:构建大鼠肝硬化模型,并采用骨髓间充质干细胞移植治疗,透射电镜观察大鼠肝组织的超微结构,体视学分析软件分析模型组、治疗组、正常组大鼠肝窦的体视学参数变化,生化仪检测肝功能指标的改变。结果与结论:1模型大鼠肝细胞缺氧严重,许多线粒体破坏,核固缩明显,肝窦呈毛细血管化;骨髓间充质干细胞治疗组肝组织超微结构明显改善,肝细胞核基本正常,线粒体肿胀显著缓解,核膜孔堵塞现象有所减轻。2模型组大鼠肝窦数目明显减少,而肝窦总面积及平均直径明显增大,与治疗组及正常组比较差异有显著性意义(P<0.05)。3与模型组比较,治疗组肝功能明显改善,白蛋白水平显著升高,谷丙转氨酶及直接胆红素水平显著下降(P<0.05),但谷草转氨酶变化不明显(P>0.05)。4结果提示骨髓间充质干细胞移植可改善肝硬化大鼠肝功能和肝组织结构,减轻肝窦病变,对肝硬化有一定的治疗作用。BACKGROUND： Mesenchymal stem cell transplantation has a certain controversy in the treatment of liver cirrhosis, and its effects on the receptor liver structure and function need further studies. OBJECTIVE： To study the changes in liver ultrastructure, stereology parameters and liver function indexes of rat models with liver cirrhosis treated by bone marrow mesenchymal stem cell transplantation. METHODS： Rat models of liver cirrhosis were made using carbon tetrachloride and treated by bone marrow mesenchymal stem cell transplantation. Liver ultrastructure of all the rats were observed by transmission electron microscope, the stereology parameters of the hepatic sinusoid were analyzed by a stereology analysis software, and the serum liver function indexes were detected by a biochemical analyzer. RESULTS AND CONCLUSION：（1） The hepatic cells in the rat models exhibited acute hypoxia, lots of mitochondria were destroyed, and obvious karyopycnosis and capillarization of the hepatic sinusoid were found. The liver ultrastructure of rats undergoing cell transplantation was improved remarkably, the hepatic cell nucleus was nearly normal, mitochondrial swelling relieved notably and nuclear pore clogging lessened.（2） The number of hepatic sinusoids in the model group was reduced dramatically, but the total area and mean diameter of the hepatic sinusoid were enlarged significantly as compared with the cell transplantation and normal groups（P〈0.05）.（3） Compared with the model group, the albumin level was significantly increased, but the levels of alanine aminotransferase and direct bilirubin were significantly decreased in the cell transplantation（P〈0.05）. In addition,there was no significant difference in the level of aspartate aminotransferase between the model and cell transplantation groups（P〉0.05）. These findings indicate that bone marrow mesenchymal stem cell transplantation can improve liver function and structure of rats with liver cirrhosis and lessen pathological changes of hepa

关 键 词：肝硬化 骨髓 间质干细胞移植 组织工程 干细胞 移植 超微结构 骨髓间充质干细胞 体视学 肝功能 

分 类 号：R394.2[医药卫生—医学遗传学]