p16、p53、Ki-67和血清SCC-Ag在宫颈鳞癌中的表达及意义  

Expression of p16,p53,Ki-67 and serum SCC-Ag levels in cervical squamous cell carcinoma

作　　者：吕嘉[1] 冯砯锦 钱春妹[1] 王锦波[1] 曹梅[1] 成佳景[1] 

机构地区：[1]同济大学附属第十人民医院妇产科,上海200072 [2]同济大学附属第十人民医院病理科,上海200072

出　　处：《同济大学学报（医学版）》2015年第6期50-55,共6页Journal of Tongji University(Medical Science)

摘　　要：目的探讨p16、p53、Ki-67和血清鳞状细胞癌抗原(squamous cell carcinoma antigen,SCC-Ag)在宫颈鳞癌患者中的表达及意义。方法采用免疫组织化学法检测31例宫颈鳞癌与31例宫颈炎组织中p16、p53、Ki-67的表达,同时采用化学发光微粒子免疫法检测治疗前的血清SCC-Ag水平。结果 (1)宫颈炎和宫颈鳞癌中p16、p53、Ki-67、SCC-Ag的阳性率分别为0%、0%、12.9%、0%和96.8%、48.4%、100%、71%,两组比较有显著差异(P<0.05)。(2)p53高表达与淋巴结或脉管转移有关,Ki-67表达随组织学分级升高而升高,血清SCC-Ag水平随临床分期升高而升高(P<0.05)。(3)经Spearman等级相关分析,p16染色强度与血清SCC-Ag水平呈正相关(r=0.467,P<0.05),在临床Ⅰ期宫颈鳞癌中,两者间正相关更显著(r=0.59,P<0.05)。结论 P53、Ki-67、血清SCC-Ag与宫颈鳞癌发展的恶性程度相关,其在表达上的差异与肿瘤生物学行为有一定关系,宫颈鳞癌在发生发展过程中,尤其是早期阶段,p16与血清SCC-Ag可能有协同作用。Objective To investigate the expression of p16,p53,Ki-67 and the serum level of squamous cell carcinoma antigen SCC-Ag in cervical squamous cell carcinoma and its significance.Methods The tissue expression of p16,p53,Ki-67 was detected by immunohistochemistry,and serum SCC-Ag level was measured by chemiluinescent microparticle immunoassay in 31 cases of cervical squamous cell carcinoma and 31 cases of cervicitis. Results The positive rates of p16,p53,Ki-67,SCC-Ag in cervicitis and cervical squamous cell carcinoma were 0%,0%,12. 9%,0% and96. 8%,48. 4%,100%,71%,respectively; tere were significant differences between two groups（ P 0. 05）. The strong expression of p53 was correlated with lymph node or vasculature metastasis.The expression of Ki-67 was increased with raising of histologic grades. The serum SCC-Ag level was increased with increasing of FIGO stages（ P 0. 05）. Spearman correlation analysis showed that p16 expression was positively correlated with serum SCC-Ag level in cervical squamous cell carcinoma（ r =0. 467,P 0. 05）; and the Spearman correlation coefficient was higher（ r = 0. 59,P 0. 05） in FIGO stage I. Conclusion p53,Ki-67 and serum SCC-Ag may be associated with the malignant degree and biological behavior of cervical squamous cell carcinoma.

关 键 词：宫颈鳞肿瘤 P16 p53 KI-67 SCC-AG 

分 类 号：R737.33[医药卫生—肿瘤]