石杉碱甲与银杏内酯B联合用药跨细胞转运研究  

Study on transcellular transport of huperzine A and ginkgolide B

作　　者：张琦[1,2] 赵文萃 郭涛[2] 刘福强 

机构地区：[1]解放军第二〇八医院药剂科,吉林长春130062 [2]沈阳军区总医院,辽宁沈阳110016

出　　处：《临床军医杂志》2015年第5期540-543,545,共5页Clinical Journal of Medical Officers

基　　金：国家自然科学基金项目(81173559)

摘　　要：目的利用Caco-2和MDCK细胞单层模型探讨石杉碱甲与银杏内酯B的跨细胞转运过程及其机制。方法建立Caco-2和MDCK细胞单层模型,将Caco-2和MDCK细胞分别按密度1×105、5×104个细胞/cm2接种到transwell culture plate inserts上培养,待细胞单层达到一定密度程度后进行转运实验。用高效液相色谱法测定石杉碱甲和银杏内酯B在不同侧面、不同浓度条件的跨单层细胞转运的情况,同时计算累计的透过量。结果在两种不同单层细胞模型上,石杉碱甲与银杏内酯B都有不同程度的吸收和分泌。结论石杉碱甲和银杏内酯B跨细胞转运同时存在吸收和分泌的动力学过程。Objective To study the transcellular transport of huperzine A and Ginkgolide B. Methods After Caco-2 and MDCK cell monolayer was set up,Caco-2 and MDCK cells（ at a density of 1 × 10^5 and 5 × 10^4 cells / cm2） were inoculated onto Transwell Culture Plate Inserts for culture. Transcellular transport tests of huperzine A and ginkgolide B across monolayer were performed. Results There were some absorption and secretion of huperzine A and ginkgolide B across the two kinds of monolayers. Conclusion There are simultaneous dynamic actions of absorption and secretion during transcellular transport of huperzine A and ginkgolide B.

关 键 词：CACO-2 MDCK 石杉碱甲 银杏内酯B 跨细胞转运 

分 类 号：R749.16[医药卫生—神经病学与精神病学]