牛磺酸对鲫鱼(Carassius auratus)生长、肠道细胞增殖及蛋白消化吸收相关基因表达的影响  

PROPER TAURINE-ENRICHED FISHMEAL PROMOTES GROWTH OF CRUCIAN CARP CARASSIUS AURATUS

作　　者：伍琴[1,2] 唐建洲[2] 刘臻[3,2] 赵玉蓉[1] 郝光[2] 鲁双庆[2] 

机构地区：[1]湖南农业大学动物科技学院,长沙410128 [2]长沙学院生物与环境工程系,长沙410003 [3]湖南省水产高效健康生产协同创新中心,常德415000

出　　处：《海洋与湖沼》2015年第6期1516-1523,共8页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金项目;31272675号;湖南省教育厅优秀青年项目;13B144号;湖南省高校产业化培育项目;13CY031号

摘　　要：本文探讨牛磺酸在鲫鱼生长、肠道细胞增殖分化以及蛋白质消化吸收相关基因的表达等方面的作用。试验以480尾[初始体质量(29.07±0.19)g]鲫鱼(Carassius auratus)为研究对象,随机分成6组,每组设定4个重复,每个重复有鱼20尾。鲫鱼基础饲料中添加的牛磺酸的剂量分别是0.0、1.0、2.5、4.0、6.0以及9.0 g/kg,以0.0 g/kg添加组为对照组,试验持续8周。结果表明:(1)牛磺酸对鲫鱼有促生长作用,6.0 g/kg添加组的鱼的增重率,特定生长率,蛋白质效率,饲料系数等都显著高于对照组(P<0.05)。(2)牛磺酸能促进肠绒毛的生长,降低隐窝深度。(3)牛磺酸提高了鲫鱼血清中T3、T4的含量,添加6.0 g/kg时显著高于对照组,且此时血清尿素氮浓度显著低于对照组和其它处理组(P<0.05)。(4)氨肽酶N(APN)、小肽转运蛋白(Pep T1)、基因系尾型同源盒基因(CDX2)、L-氨基酸转运载体蛋白(LAT2)等基因的相对表达量随牛磺酸剂量增加而上调,6.0 g/kg时显著高于对照组和其它实验组(P<0.05)。由此可见,适量添加牛磺酸能影响鲫鱼生长、肠道细胞增殖及蛋白消化吸收相关基因的表达。To study the effect of taurine-enriched feed on growth of Carassius auratus, taurine in different concentrations（0.0, 1.0, 2.5, 4.0, 6.0, and 9.0 g/kg） was tested in 8 weeks, during which proliferation of intestinal cell and expressions of genes associated with protein digestion and absorption were studied. A total of 480 individuals in body weight of 29.07±0.19 g were selected and randomly divided into 6 treatments containing 4 replicates each with 20 individuals. The basal diet with no taurine was used as the control. The results show that taurine could promote the growth performance best at 6.0 g/kg, at which the weight gain rate, specific growth rate, feed coefficient, and protein efficiency ratio increased remarkably than the control（P〉0.05）. In specific, taurine-addition promoted the growth of intestinal villus, reduced crypt depth remarkably, enhanced the serum T3 and T4 concentrations, and reduced serum urea nitrogen concentration significantly（P〉0.05）. In addition, taurine enhanced remarkably the relative expression of APN, Pep T1, LAT2, and CDX2 m RNA. Therefore, a proper amount of taurine supplementation is necessary for the proliferation of intestinal cell, genes associated with protein digestion and absorption of the fish, favoring the growth performance.

关 键 词：牛磺酸 鲫鱼 生长 蛋白质 细胞增殖 

分 类 号：S963[农业科学—水产养殖]