胃铋镁颗粒对阿司匹林所致大鼠胃黏膜损伤的预防作用机制  

Protective effect of compound bismuth and magnesium granules on aspirin-induced gastric mucosal injury in rats

作　　者：牟方宏[1] 胡伏莲[2] 魏红[1] 张洋阳[3] 杨桂彬[1] 雷晓燕[3] 杨友鹏 孙伟娜 崔梅花[1] 

机构地区：[1]航天中心医院北京大学航天临床医学院消化科,100049 [2]北京大学第一医院消化科 [3]辽宁医学院校外培养基地航天中心医院消化科 [4]北京门头沟区医院急诊科

出　　处：《中华医学杂志》2016年第6期464-467,共4页National Medical Journal of China

基　　金：中华医学会北京分会科研发展基金（2012001）

摘　　要：目的 研究胃铋镁颗粒对阿司匹林所致大鼠胃黏膜损伤的预防保护作用及其机制.方法 采用阿司匹林腹腔注射造成Wistar大鼠急性胃黏膜损伤模型.实验动物分为正常对照组、阿司匹林损伤组、硫糖铝保护组、胃铋镁保护组和胃铋镁植物药组分保护组,每组12只.保护组分别用硫糖铝、胃铋镁和胃铋镁植物药组分灌胃,其后用阿司匹林行腹腔注射,然后分别测定各组的胃黏膜损伤指数、胃黏膜血流、胃黏膜氨基己糖、前列腺素（PG）、一氧化氮（N0）、肿瘤坏死因子（TNF）、白细胞介素（IL）1、2、8含量,并观察大体及显微镜下及电子显微镜下组织学变化,以评价胃黏膜损伤程度及药物的预防保护效果.结果 各保护组大鼠胃黏膜氨基己糖、NO、PG含量明显高于阿司匹林损伤组（均P＜0.01）,并且胃铋镁保护组NO含量高于硫糖铝保护组[（11.29±0.51）比（10.80±0.36）nmol/ml,P＜0.05];各保护组黏膜损伤指数、TNF、IL-1、2、8含量显著低于阿司匹林损伤组（均P＜0.01）,并且胃铋镁保护组IL-2、IL-8含量显著低于硫糖铝保护组[（328.17±6.56）比（340.23±8.05） pg/ml,P＜0.01;（170.82 ±7.31）比（179.31±7.80）pg/ml,P＜0.05].镜下,各保护组胃黏膜组织损伤、炎症反应均较阿司匹林损伤组明显减轻.结论 胃铋镁及其植物药组分对阿司匹林所致胃黏膜损伤有明显保护作用.Objective To investigate the protective effect of compound bismuth and magnesium granules on aspirin-induced gastric mucosal injury in rats and its possible mechanism.Methods Acute gastric mucosal injury model was developed with intraperitoneal injection of aspirin in Wistar rats.The rats were divided into normal control group,injury group,sucralfate protection group,compound bismuth and magnesium granules protection group and its herbal components protection group（each group 12 rats）.In the protection groups,drugs as mentioned above were administered by gavage before treated with intraperitoneal injection of aspirin.To evaluate the extent of gastric mucosal injury and the protective effect of drugs,gastric mucosal lesion irndex,gastric mucosal blood flow,content of gastric mucosal hexosamine,prostaglandins （PG）,nitric oxide（NO）,tumor necrosis factor （TNF）,and interleukin （IL）-1,2,8 were measured in each group,and histological changes were observed by gross as well as under microscope and electron microscope.Results Contents of hexosamine,NO,and PG in all the protection groups were significantly higher than those in the injury group （all P 〈 0.01）,and content of NO in the compound bismuth and magnesium granules group was significantly higher than that in the sucralfate group （（11.29 ± 0.51） vs （10.80 ±0.36）nmol/ml,P 〈0.05）.The gastric mucosal lesion index,contents of TNF,and IL-1,2,8 were significantly lower in all the protection groups than in the injury group （all P 〈 0.01）,and contents of IL-2 and IL-8 in the compound bismuth and magnesium granules group were significantly lower than those in the sucralfate group （（328.17 ± 6.56） vs （340.23 ± 8.05） pg/ml,P 〈 O.01;（170.82 ± 7.31） vs （179.31 ±7.80） pg/ml,P 〈0.05）.Tissue injury and inflammatory reaction in all the protection groups were obviously mitigated compared with the injury group.Conclusion Compound bismuth and magnesium granules and its herbal components may have significant prote

关 键 词：阿司匹林 胃黏膜 胃铋镁颗粒 

分 类 号：R573[医药卫生—消化系统]