小叶女贞总黄酮的提取及对橄榄保鲜效果的研究  

Extraction of Total Flavonoids from Ligustrum quihoui Car and Its Preservative Effects on Chinese Olive

作　　者：周相助 叶清华[1] 张舒婷[1] 陈清西[1] 钟凤林[1] 

机构地区：[1]福建农林大学园艺学院,福州350002

出　　处：《天然产物研究与开发》2016年第1期102-106,共5页Natural Product Research and Development

基　　金：国家科技支持计划(2007BAD07B01);福建省科技重大专项(2013NZ0002-4);福建省自然科学基金(2012D085);福州市科技计划(2009-N-55)

摘　　要：本试验研究小叶女贞叶片总黄酮的高效提取方法及其对橄榄果实的保鲜效果,通过单因素和正交试验,得出用超声法提取总黄酮的最佳工艺条件,并将总黄酮分别稀释成2、4、8倍用于橄榄的涂膜保鲜。得出总黄酮最佳提取工艺条件为液固比25∶1(m L/g)、功率540 W、乙醇浓度60%、提取时间4 h,提取得率为4.71%;橄榄经总黄酮处理69 d后,好果率最高为稀释8倍、其次是4倍且与对照差异显著,2倍显著低于对照,另外,橄榄鲜食品质也均好于对照,可溶性总糖含量下降延缓,糖酚比提高,多酚含量下降加速。研究结果为利用小叶女贞总黄酮提供了试验依据。In this study,an efficient method for the extraction of total flavonoids from the leaves of Ligustrum quihoui was developed,the preservative effects of the extracted flavonoids on Chinese olives were investigated. The extraction conditions were optimized by single factor and orthogonal experiments. The 2 times,4 times and 8 times diluents of the extracted flavonoids,were subjected to fresh Chinese olives. The experimental results showed that the optimal extraction conditions were liquid-solid ratio of 25∶ 1（ m L / g）,ultrasonic power of 540 W,ethanol concentration of 60%,extraction time of 4 h,under these conditions,the extraction yield of flavonoids from L. quihoui was 4. 71%. The Chinese olives were dealt after 69 days by the total flavonoids diluents. The experimental results showed that the highest rate of good fruit was 8 times diluent,followed the 4 times diluent. They were significantly superior to that of control,but the good fruit rate of 2 times diluent was significantly inferior to that of control. In addition,the quality of fresh-eating was also significantly superior to that of control. The decreasing of soluble total sugar content was delayed,the ratio of polyphenol and sugar was increased,and the content of polyphenol was slowly declined. These results can be applied for the analysis of total flavonoids from the leaves of L. quihoui.

关 键 词：小叶女贞 总黄酮 橄榄 保鲜 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]