基于PLFA法分析亚硝氮、硝氮和氨氮对厌氧微生物细菌群落的影响  

Effects of nitrite, nitrate and ammonia nitrogen on anaerobic microbial community characterized by using phospholipid fatty acid PLFA method

作　　者：周莉娜[1] 苏润华[1] 马思佳[1] 何甦[1] 任洪强[1] 丁丽丽[1] 

机构地区：[1]南京大学环境学院,污染控制与资源化研究国家重点实验室,南京210023

出　　处：《环境科学学报》2016年第2期499-505,共7页Acta Scientiae Circumstantiae

基　　金：国家科技支撑计划(No.2014BAC08B04)~~

摘　　要：利用磷脂脂肪酸(PLFA)技术研究了亚硝氮、硝氮和氨氮在不同浓度水平下对中温厌氧颗粒污泥中厌氧细菌群落结构的影响.结果表明,高浓度亚硝氮(360 mg·L-1,以N计)、硝氮(300 mg·L-1,以N计)和氨氮(3000 mg·L-1,以N计)使系统COD去除率分别下降至3.49%、10.85%和71.53%,并使污泥表面主要细菌由杆菌变为球状菌;亚硝氮和硝氮引起厌氧细菌和革兰氏阳性菌的含量下降,氨氮引起革兰氏阴性菌的含量下降;PLFA香农-威尔多样性指数随着亚硝氮和硝氮浓度的升高从1.12分别下降至0.96和1.03,氨氮对PLFA香农-威尔多样性指数无明显影响.Phospholipid fatty acid（ PLFA） method was used to investigate the effects of nitrite,nitrate and ammonia nitrogen on microbial community of anaerobic granular sludge under mesophilic condition. Results showed that high concentration of nitrite（ 360 mg（ N）·L- 1）,nitrate（ 300 mg（ N）·L- 1）and ammonia（ 3000 mg（ N）·L- 1） decreased the COD removal rates to 3. 49%,10. 85% and 71. 53% respectively,and the dominant bacteria on the sludge surface changed from Bacillus to Sphaerophorus. PLFA biomarkers showed that the content of anaerobic bacteria and Gram-positive bacteria decreased with the addition of nitrite and nitrate,and the content of Gram-negative bacteria declined with the addition of ammonia. High concentration of nitrite and nitrate lead the PLFA Shannon-Wiener diversity index decreased from 1. 12 to 0. 96 and 1. 03,respectively. While,ammonia nitrogen had no effect on the PLFA Shannon- Wiener diversity index of anaerobic microorganism.

关 键 词：亚硝氮 硝氮 氨氮 厌氧细菌群落 磷脂脂肪酸 

分 类 号：X172[环境科学与工程—环境科学]