纳米羟基磷灰石表面阿伦膦酸钠和I型胶原蛋白复合膜的制备  

Formation of Alendronate Sodium and Collagen Type I Complex Films on the Nano-hydroxyapatite Surface

作　　者：姜燕 姚江武 

机构地区：[1]厦门市口腔医院修复科,福建厦门361003

出　　处：《口腔医学研究》2016年第1期21-25,共5页Journal of Oral Science Research

基　　金：福建省自然科学基金面上项目(编号:2013D010)

摘　　要：目的:在纳米羟基磷灰石(nHA)涂层表面制备稳定可重复的阿伦膦酸钠(ALN)和I型胶原蛋白(COL I)复合膜。方法:在纯钛(PT)表面电化学沉积nHA涂层,以3-氨丙基三乙氧基硅烷(APTES)、1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐(EDC)为偶联剂,在涂层表面结合ALN和COL I,制备nHA/ALN/COL I和nHA/COL I/ALN复合膜。通过扫描电子显微镜(SEM),X射线能谱分析仪(EDS)和X射线衍射仪(XRD)表征nHA涂层,通过X射线光电子能谱仪(XPS)和傅里叶红外光谱仪(FTIR)表征复合膜。结果:ALN和COL I可通过偶联剂结合于nHA涂层,改变其结合次序,可分别形成nHA/ALN/COL I和nHA/COL I/ALN复合膜。结论:通过化学结合法形成的nHA/ALN/COL I和nHA/COL I/ALN复合膜具有不同的ALN缓释潜力。Objective： To form a stable and repeatable alendronate sodium （ALN） and collagen type I （COL I） complex films on nano-- hydroxyapatite （nHA） layer. Methods： A nHA layer was coated on the pure titanium （PT） surface by using electrochemical deposition, then ALN and COL I were chemically bonded on the nHA layer to form nHA/ALN/COL I and nHA/COL I/ALN complex films using 3--aminopropyl triethoxysilane （APTES） and 1--（3--Dimethylaminopropyl）- 3--ethylcarbodiimide hydrochloride （EDC） as coupling agent. The nHA layer was characterized by scanning electron microscope （SEM）, energy, dispersive X--ray spectroscopy （EDS） and X-- ray diffractometer （XRD）, and the complex films were characterized by X--ray photoelectron spectrometer （XPS） and Fourier transform infrared spectroscopy （FTIR）. Results： ALN and COL I were bonded on the nHA layer by coupling agent, nHA/ALN/COL I and nHA/COL I/ALN complex films were formed by changing the binding or- der of ALN and COL I. Conclusion： The nHA/ALN/COL I and nHA/COL I/ALN complex films formed by chem- ical covalent bonding method have different potentials of ALN sustained--release.

关 键 词：纳米羟基磷灰石 阿伦膦酸钠 I型胶原蛋白 表面修饰 

分 类 号：R783.1[医药卫生—口腔医学]