一锅法合成近红外量子点用于胶质瘤荧光显像与光动力治疗  

Near-infrared quantum dots through one-pot aqueous synthesization for bio-imaging and photodynamic therapy of gliomas

作　　者：刘明兴[1] 仲骏[1] 窦宁宁[1] 夏磊[1] 高国[2] 

机构地区：[1]上海交通大学医学院附属新华医院神经外科,200092 [2]上海交通大学纳米生物医学工程研究所薄膜与微细技术教育部重点实验室,200000

出　　处：《实用医学杂志》2016年第1期29-33,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81471317);上海市科学技术委员会引导类重点项目(编号:124119a0800)

摘　　要：目的:合成近红外量子点并验证其荧光成像和光动力效应。方法:应用一锅法水相合成近红外量子点CdTe,经表征检测后连接RGD多肽,再用激光共聚焦显微镜观察其荧光成像效果。随后,将3~24μg/mL的CdTe-RGD分别加入U251和3T3细胞,避光培养24 h间,观察量子点的细胞毒性。最后用强度为30 mW/cm^2,波长为632.8 nm的激光照射量子点处理后的细胞5~60 min,观察其光动力效应。结果:合成的CdTe具有良好的晶体结构和分散性,其直径为3.75 nm,发射波长700 nm,荧光产率20%。经量子点处理后,U251细胞可荧光显像而3T3细胞则不能。暗环境下,不同浓度CdTe-RGD处理过的U251和3T3细胞存活率都在85%以上。激光照射后,随光照时间延长和量子点浓度的增加,U251细胞存活逐渐下降至(37±1.6)%。而3T3细胞组的存活率则无明显变化。结论:一锅法水相合成的CdTe-RGD具有良好的理化性质且细胞毒性低,在生物成像和光动力治疗方面具有应用前景。Objective To synthesize a quantum dot（QD） to recognize glioma cells for imaging and photodynamic therapy.Methods By one-pot aqueous approach,near infrared-emitting CdTe was produced.After detection of its physicochemical characterizations,RGD was conjugated.Emission images were observed with confocal microscopy.To test its toxicity,CdTe-RGD with various concentrations was separately added into U251 and 3T3 cells for incubation in dark circulation.To test its photodynamic effect,U251 and 3T3 cells were then irradiated for 5-60 min using 632.8 nm laser.Results The QD（Φ=3.75 nm,PL peak wavelength=700 nm,PLQY = 20%） achieved was a spherical crystal with excellent monodispersity.Under confocal microscope,U251 cells were visualized but 3T3 cells not.In dark circulation,the survival rates of both U251 and 3T3 cells were above 85%.After laser irradiation,the survival rate of U251 cells decreased to（37 ± 1.6）%with the increasing of irradiation time and CdTe-RGD concentration.Conclusion With good physicochemical characterization and low toxicity,CdTe-RGD could be applied in biomedical imaging and photodynamic therapy of gliomas.

关 键 词：胶质瘤 近红外 量子点 生物成像 光动力治疗 

分 类 号：R739.41[医药卫生—肿瘤]