同时性结直肠癌肝转移结直肠癌组织中Kiss-1蛋白和基质金属蛋白酶9蛋白的表达及意义  

Expressions and significances of Kiss-1 and matrix metalloproteinases-9 proteins in the colorectal cancer tissues of patients with synchronous colorectal liver metastasis

作　　者：惠菲菲[1] 刘凯[2] 杨家和[2] 高鹏[1] 杨彬[1] 

机构地区：[1]济宁市第一人民医院普通外科,272000 [2]第二军医大学附属东方肝胆外科医院腹腔镜科,上海200438

出　　处：《中华消化外科杂志》2016年第2期153-160,共8页Chinese Journal of Digestive Surgery

基　　金：国家自然科学基金（81070359）

摘　　要：目的 探讨同时性结直肠癌肝转移（SCRLM）患者结直肠癌组织中Kiss-1蛋白和MMP9蛋白的表达及其与SCRLM患者临床病理因素和预后的关系,以及两者表达的相关性.方法 采用回顾性病例对照研究方法.收集2000年1月至2013年5月第二军医大学附属东方肝胆外科医院收治的96例SCRLM患者和69例无转移结直肠癌患者的临床病理资料.收集96例SCRLM患者手术切除的结直肠癌组织及其中50例癌旁组织（距离阴性切缘≥5 cm）;69例无转移结直肠癌患者手术切除的结直肠癌组织进行研究.采用免疫组织化学染色检测Kiss-Ⅰ蛋白和MMP9蛋白的表达情况.通过门诊和电话方式随访患者的生存情况,随访时间截至2014年8月.计数资料的比较及SCRLM患者结直肠癌组织中Kiss-1蛋白和MMP9蛋白表达与患者临床病理因素的关系采用x2检验或Fisher确切概率法.采用Kaplan-Meier法绘制生存曲线,采用Log-rank检验进行生存分析.相关性分析采用Pearson等级相关分析.结果 Kiss-1蛋白的表达位于组织细胞的细胞质,其阳性表达率在SCRLM患者结直肠癌组织、无转移结直肠癌患者的结直肠癌组织、SCRLM患者结直肠癌旁组织中分别为24.0％（23/96）、43.5％（30/69）、52.0％ （26/50）,3者比较,差异有统计学意义（x2=14.307,P＜0.05）.其中SCRLM患者结直肠癌组织中Kiss-1蛋白的阳性表达率分别与无转移结直肠癌患者的结直肠癌组织和SCRLM患者结直肠癌旁组织比较,差异均有统计学意义（x2=0.702,11.594,P＜0.05）;无转移结直肠癌患者的结直肠癌组织与SCRLM患者结直肠癌旁组织比较,差异无统计学意义（x2=0.845,P＞0.05）.MMP9蛋白的表达位于组织细胞的细胞质,其阳性表达率在SCRLM患者结直肠癌组织、无转移结直肠癌患者的结直肠癌组织、SCRLM患者结直肠癌旁组织中分别为67.7％ （65/96）、62.3％ （43/69）、36.0％ （18/50）,3者比较,差异有统计学意义（x2Objective To investigate the expressions and correlations of Kiss-1 and matrix metalloproteinases-9 （MMP9） proteins in the colorectal cancer （CRC） tissues of patients with synchronous colorectal liver metastasis （SCRLM）,and the association with the clinicopathologic factors and prognosis of patients.Methods The retrospective case-control study was adopted.The clinicopathological data of 96 patients with SCRLM and 69 patients with CRC and no metastasis who were admitted to the Eastern Hepatobiliary Surgery Hospital of the Second Military Medical University from January 2000 to May 2013 were collected.The 96 CRC tissues and 50 adjacent normal tissues （distance from resection margin ≥ 5 cm） were collected from 96 patients with SCRLM,and 69 CRC tissues were collected from 69 patients with CRC and no metastasis.Expressions of Kiss-1 and MMP9 protein were detected by immunohistochemistry （IHC）.The follow-up of outpatient examination and telephone interview was performed to detect survival of patients till August 2014.Comparison of count data and correlation between expressions of Kiss-1 or MMP9 protein and clinicopathological factors were analyzed by the chi-square test and Fisher exact probability.Survival curve was drawn using the Kaplan-Meier method,and survival analysis was done using the Log-rank test.Correlation analysis was done by the Pearson correlation.Results Expression of Kiss-1 protein was located in the cytoplasm of tissue cells.The positive expression rates of Kiss-1 protein in CRC tissues of patients with SCRLM and with CRC and no metastasis and in adjacent normal tissues were 24.0％ （23/96）,43.5％ （30/69） and 52.0％ （26/50）,respectively,with a significant difference among the 3 tissues （x2 =14.307,P ＜ 0.05） and no significant difference between CRC tissues of patients with CRC and no metastasis and patients with SCRLM （x2 =0.845,P ＞ 0.05）.The positive expression rate of Kiss-1 protein in CRC tissues of patients with SCRLM was significantly different from

关 键 词：结直肠肿瘤 肝转移 同时性 KISS-1 基质金属蛋白酶9 预后 

分 类 号：R735.34[医药卫生—肿瘤]