低表达miRNA-21对人肺癌H358细胞增殖及凋亡作用的研究  

Study on effects of low expression of miRNA-21 on proliferation and apoptosis of human lung cancer H358 cell

作　　者：黄军霞 李颖 钭建波 涂应锋[2] 

机构地区：[1]浙江省衢州市开化县人民医院内科,浙江衢州324300 [2]哈尔滨医科大学附属第四临床医院心内科,黑龙江哈尔滨150001

出　　处：《重庆医学》2016年第5期588-591,共4页Chongqing medicine

基　　金：国家自然科学基金青年科学基金资助项目(81401457);中国国家博士后基金面上项目(2014M561376);黑龙江省博士后资助经费(LBH-Z14143);黑龙江省自然科学基金留学基金(LC2015038);黑龙江省卫生计生委面上项目(2014-385)

摘　　要：目的探讨微RNA(miRNA)-21对人肺癌H358细胞体外增殖及诱导凋亡方面的作用。方法采用体外转染法将miRNA-21抑制剂瞬时转染H358细胞48h后,应用定量real-time PCR特异探针法检测H358细胞中miRNA-21的表达水平,应用MTT法检测H358细胞的生长情况,荧光缺口末端标记法(TUNEL)法分析H358细胞凋亡情况,并用Western blot检测H358细胞中抑癌基因PTEN表达的变化。结果与对照组比较,miRNA-21抑制剂转染组H358细胞的miRNA-21表达水平显著降低(P<0.05),细胞生长明显受到抑制(P<0.05),且通过PTEN靶蛋白诱导细胞凋亡(P<0.05)。结论低表达miRNA-21可以抑制人肺癌H358细胞的体外增殖,诱导细胞凋亡,为肺癌生物治疗提供新的治疗靶点。Objective To study the effects of microRNA-21 on the proliferation and apoptosis of human lung cancer cell line H358.Methods MiRNA-21 inhibitor was transiently transferred into human lung cancer cell line H358 by using transfection in vitro.The expression level of miRNA-21 in H358cells was detected by real-time PCR before or after transfection.The MTT assay was used to assess the proliferation of H358 cells.And the cell apoptosis was analyzed by TUNEL Apoptosis Assay Kit.Furthermore,the expression of phosphatase and tensin homolog deleted on chromosome ten（PTEN）was determined by the Western-blot assay.Results The expression of miRNA-21 was markedly decreased compared with the control group（P〈0.05）.The proliferation of miRNA-21inhibitor-tranfected H358 cells was obviously inhibited（P〈0.01）.And expression level of miRNA-21 in H358cells was significantly decreased,the cell growth was significantly inhibited,moreover apoptosis was induced by PTEN target protein（P〈0.05）.Conclusion Low expression of miRNA-21 could inhibit the in vitro proliferation of H358 cells and induces apoptosis,which could provide a novel therapeutic target for anti-cancer biotherapy.

关 键 词：肺肿瘤 微RNA PTEN 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]