Cloning and Bioinformatics Analysis of zmPKC  

蛋白激酶C的克隆和生物信息学分析（英文）

作　　者：纪艺红 张彦彦[2] 潘金豹[2] 于涌鲲[2] 卢敏[2] 韩俊[2] 南张杰[2] 孙清鹏 

机构地区：[1]农业应用新技术北京重点实验室,北京102206 [2]北京农学院植物科学技术学院,北京102206

出　　处：《Agricultural Science & Technology》2016年第1期42-44,160,共4页农业科学与技术（英文版）

基　　金：Supported by Beijing Natural Science Foundation(5132006)~~

摘　　要：[Objective] Protein kinase C（PKC） plays a vital role in signal transmission,stomatal regulation, cell proliferation and differentiation. This study was to clone zm PKC and predict its characteristics by bioinformatics. [Method] The c DNA of zea mays protein kinase C（zm PKC） gene was cloned from B73 by RT-PCR, and its characteristics were predicted with bioinformatics methods, including conserved domains, physical and chemical parameters, specific phosphorylation sites and N-Glycosylation potential sites. [Result] The zm PKC had a size of 1 269 bp, and encoded 422 amino acids with 1 extron and 2 introns, containing a PKC domain, two NGlycosylation potential sites and 28 kinase specific phosphorylation sites. Furthermore, the prediction of physical and chemical parameters revealed that the isoelectric point was 4.98 and molecular weight was 132 074.70. [Conclusion] Cloning and bioinformatics analysis of zm PKC gene laid a foundation for further analysis of the functions of the PKC.[目的]PKC在信号转导、气孔调节、细胞分化中具有重要作用。该研究目的是为了克隆蛋白激酶C并预测它的性质。[方法]从玉米B73中克隆得到zm PKC(玉米蛋白激酶C)的c DNA,并且通过生物信息学的方法预测了它的特性,包括保守结构域、理化性质、特殊磷酸化位点和N端糖基化位点等。[结果]证明zm PKC长1 269 bp,编码422个氨基酸,有一个外显子和俩个内含子及一个蛋白激酶域,2个N端糖基化位点和28个特殊磷酸化位点,等电点为4.98,分子量为132 074.70。[结论]zm PKC的克隆和生物信息学分析能利于进一步研究PKC的功能。

关 键 词：Zea mays zm PKC BIOINFORMATICS 

分 类 号：Q943.2[生物学—植物学]