异养硝化-好氧反硝化菌Burkholderia sp.YX02强化连续流反应器中微生物群落结构解析  

Analysis of the Microbial Community Structure in Continuous Flow Reactor Enhanced by Heterotrophic Nitrification and Aerobic Denitrification Bacterium Burkholderia sp. YX02

作　　者：邵基伦 曹刚[1,2] 李紫惠 黄郑郑 罗恺[1] 莫测辉[1,2] 

机构地区：[1]暨南大学环境学院.广州510630 [2]广东省污染控制与修复材料工程技术中心,广州510630

出　　处：《环境科学》2016年第2期630-637,共8页Environmental Science

基　　金：广东省科技计划项目(2011B030800009)

摘　　要：为揭示生物脱氮反应器中微生物群落结构随时间变化的动态演替过程,以投加包埋固定化异养硝化-好氧反硝化菌Burkholderia sp.YX02建立的连续流反应器为研究对象,利用PCR-DGGE技术获得了不同阶段下微生物群落结构的DNA特征图谱并对图谱进行了多元统计分析,同时考察了微生物群落结构的变化与反应器中p H、NH+4-N、NO-2-N、NO-3-N及COD等环境因子的相关性.结果表明,反应器在18 d的运行期间微生物群落较为丰富,各阶段群落结构的相似性并不随着反应器的运行而有序递减,相邻阶段群落结构的相似性先下降后上升,反应器内的菌群经演替最终形成了稳定的菌群结构.Shannon-Wiener多样性指数变化与反应器的运行显著关联,不同阶段多样性指数差异明显,整体上呈先下降后上升的趋势,运行后期出现了新的菌群.UPGMA聚类分析大致将反应器的运行过程划分为3个时期,且不同时期存在一定的演变关系.对DGGE图谱进行主成分分析(principal component analysis,PCA)表明,随着反应器的运行,劣势菌被大量淘汰、优势菌株常驻,运行后期形成以包埋菌Burkholderia sp.YX02为代表的新的菌群结构.对微生物的群落结构与环境因子的相关性进行典范对应分析(canonical correspondence analysis,CCA)表明,NO-2-N对微生物群落结构的影响最大,其次为NO-3-N、NH+4-N和COD,p H影响最小.To reveal the dynamic succession of microbial community structure along with time in bio-denitrification reactor,a continuous flow reactor containing immobilized heterotrophic nitrification-aerobic denitrification bacterium Burkholderia sp. YX02 was taken as a model. The microbial community structure in the bioreactor was analyzed by PCR-DGGE,and its correlations with environmental factors such as p H,NH＋4-N,NO-2-N,NO-3-N and COD were simultaneously investigated. The results showed that the microbial community was relatively rich during the early stage of 18 days. The similarity of community structure in different stages was not orderly declining with the operation. In addition,the structural similarity in adjacent stages firstly increased,then decreased,and eventually tended to be stable. Shannon-Wiener index firstly descended significantly,and then ascended with new microbial community emerging at the later stage. UPGMA clustering analysis roughly divided the process into three periods with certain relationship.Principal component analysis showed that during the operation of the bioreactor predominant bacterial community formed steadily and new microbial community dominated by Burkholderia sp. YX02 emerged at the later stage of the operation. Canonical correspondence analysis certificated that the structure of microbial community was most obviously affected by NO-2-N,followed by NO-3-N,NH＋4-N and COD,and p H had the least effect.

关 键 词：包埋固定化 异养硝化-好氧反硝化 连续流反应器 PCR-DGGE 微生物群落结构 环境相关性 

分 类 号：X172[环境科学与工程—环境科学]