桦褐孔菌内切葡聚糖酶基因的克隆与表达分析  

Cloning and Expression Analysis of Endo-1,4-β-D-glucanase Gene of Inonotus obliquus

作　　者：隋飞飞[1] 李健[1] 安丹丹[1] 陈艳秋[1] 

机构地区：[1]延边大学长白山生物资源与功能分子教育部重点实验室,延吉133002

出　　处：《吉林农业大学学报》2016年第1期38-44,共7页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(31160408)

摘　　要：利用RACE技术首次从药用真菌桦褐孔菌中克隆得到了与菌核形态发育相关的内切葡聚糖编码(EG)基因。结果表明:桦褐孔菌内切葡聚糖酶基因(IO-EG)的cDNA全长为1 315 bp,其中编码区占1 233 bp,具有13个内含子和14个外显子,共编码410个氨基酸。系统进化树分析表明,桦褐孔菌EG与嗜蓝孢孔菌EG在分子进化关系上最相近。实时荧光定量PCR分析表明,随着菌核的生长发育,IO-EG基因的表达量不断升高,初步证明内切葡聚糖酶基因的表达量与桦褐孔菌菌核的产量相关。Endoglucanase gene relative to fungal metamorphosis was first cloned from medicinal fungus Inonotus obliquus by rapid amplification of cDNA end PCR（ RACE）. The results showed that A1 315 bp full-length cDNA of endoglucanase gene contained 1 233 ORF,which was predicted to encode a 410-amino acid protein with 13 introns and 14 exons. Phylogenetic analysis showed that endoglucanase gene of I. obliquus had the closest genetic relationship with EG of Fomitiporia Mediterranea. Quantitative real-time PCR analysis revealed that IO-EG expression was increasingly higher with the development of sclerotium of I. obliquus,suggesting that IO-EG was correlated with yield of sclerotium of I. obliquus.

关 键 词：桦褐孔菌 内切葡聚糖酶 菌核 

分 类 号：Q949.329[生物学—植物学]