口蹄疫病毒O型病毒VP1蛋白表达及鉴定  

Expression and Identification of Foot and Mouth Disease Virus VP1 Protein in Escherichia coli Expression System

作　　者：李家伟[1,2] 杨勇[1] 任静强[1] 席娜[1] 张加力[2] 程世鹏[1] 郭利[1] 

机构地区：[1]中国农业科学院特产研究所,特种经济动物分子生物学省部共建国家重点实验室,长春130112 [2]吉林农业大学动物科学技术学院,长春130118

出　　处：《吉林农业大学学报》2016年第1期102-106,共5页Journal of Jilin Agricultural University

基　　金：吉林市科技计划项目(2013625020);吉林省科技发展计划项目(20130206024);中国农业科学院“创新工程”项目

摘　　要：利用大肠杆菌p ET-30a表达系统表达口蹄疫病毒(FMD)O型野毒株完整的VP1蛋白,并以Western blot试验进行鉴定。结果表明:VP1蛋白以可溶性表达,大小约为25 ku,可与标准O型口蹄疫病毒阳性血清特异结合。由此说明,口蹄疫病毒VP1原核表达蛋白有良好的反应原性,为构建口蹄疫病毒病毒样颗粒(Virus-like particles,VLPs)和快速特异诊断试剂研制奠定基础。In this study,the Escherichia coli p ET-30 a expression system was used to express the VP1 protein of foot and mouth disease（ FMD） serotype O. A wild stain of（ FMD） serotype O was chosen and a recombinant plasmid which contained complete VP1 protein（ p ET-30a-O-VP1） was constructed. The combinant VP1 was expressed in BL21,then the expressed products were purified and identified through Ni affinity chromatography and Western blot test. Western blot test indicated that the VP1 can be specifically recognized by the standard positive serum of FMD virus serotype O.The results suggested that the recombination protein of deer FMD VP1 possess a good antigenicity,which will be the basis for production of the particle like vaccine and development of a specifically diagnostic reagent of FMD.

关 键 词：口蹄疫病毒 VP1蛋白 重组质粒 

分 类 号：S852.65[农业科学—基础兽医学]