HPV分型检测在筛查宫颈癌及癌前病变中的应用  

Application of HPV type detection in screening cervical cancer and precancerous lesions

作　　者：林晴[1] 黄卓敏[1] 

机构地区：[1]深圳市妇幼保健院妇科,广东深圳518028

出　　处：《中国热带医学》2016年第1期63-65,72,共4页China Tropical Medicine

基　　金：深圳市科技创新委员会立项(No.JCYJ20150402090413004)

摘　　要：目的研究HPV分型检测在筛查宫颈癌及癌前病变中的应用价值。方法选择宫颈癌患者和宫颈上皮内瘤变患者纳入研究的宫颈癌组和CIN组,选择宫颈活检正常者纳入对照组,检测宫颈活检组织中不同分型HPV的阳性率及DNA含量、抗凋亡分子和细胞周期分子的m RNA含量。结果 CIN组和宫颈癌组患者宫颈组织中HPV-16、18的阳性率以及DNA含量高于对照组（P〈0.05）,宫颈癌组患者宫颈组织中HPV-16、18的阳性率以及DNA含量高于CIN组（P〈0.05）;三组受试者宫颈组织中HPV-31、33、51、52、58的阳性率及DNA含量无差异（P〉0.05）;CIN组和宫颈癌组患者宫颈组织中Bcl-2、Survivin、Cyclin D1、CDK1的m RNA含量高于对照组（P〈0.05）,宫颈癌组Bcl-2、Survivin、Cyclin D1、CDK1明显高于CIN组（P〈0.05）;HPV-16和HPV-18的DNA含量与Bcl-2、Survivin、Cyclin D1、CDK1的m RNA含量呈正相关（β=0.774~1.293,R=0.694~0.818,P〈0.05）。结论 HPV-16和HPV-18是造成宫颈癌及癌前病变的高危型HPV,会增加抗凋亡分子和细胞周期分子的表达。Objective To study the application value of HPV type detection in screening cervical cancer and precancerous lesions. Methods Cervical cancer and cervical intraepithelial neoplasia patients were enrolled in cervical cancer group and CIN group, normal cervical biopsy subjects were enrolled in the control group. Then the positive rate and DNA contents of different sub-types of HPV, the anti-apoptotic molecule and cell cycle molecule mRNA contents in cervical biopsy were detected. Results HPV-16, 18 positive rate and DNA contents in cervical tissue of CIN group and cervical cancer group were higher than control group （P〈0.05） ; HPV- 16, 18 positive rate and DNA contents in cervical tissue of cervical cancer group were higher than CIN group（P〈0.05） ; HPV-31, 33, 51, 52, 58 positive rate and DNA contents of three groups had no difference （P〉0.05） ;mRNA contents of Bcl-2, Survivin, Cyclin D1, CDK1 in cervical tissue of CIN group and cervical cancer group were higher than control group （P〈0.05） , mRNA contents of Bcl-2, Survivin, Cyclin D1, CDK1 in cervical cancer group were higher than CIN group （t=18.753-31.814, P〈0.05） ;HPV-16 and HPV-18 DNA contents were positively correlated with mRNA contents of Bcl-2, Survivin, Cyclin D1, CDK1 （3=0.774-1.293, R=0.694~0.818, P〈0.05）. Corlclusion HPV-16 and HPV-18 are high-risk HPV which cause the cervical cancer and precancerous lesions and will increase the expression of anti-apoptotic molecules, and cell cycle molecules.

关 键 词：宫颈癌 宫颈上皮内瘤变 人乳头瘤病毒 凋亡 增殖 

分 类 号：R737.33[医药卫生—肿瘤]