卵巢癌患者手术前后血清自体荧光光谱特征及其分子基础  

The molecular basis of the characteristics of the serum auto-fluorescent spectrum of patients with ovarian cancer and the influence of surgery on it

作　　者：乔丽华[1] 夏丽梅[1] 阎素青[2] 申延琴[3] 佴科君 马立芳[1] 江灵燕[1] 

机构地区：[1]上海市第一人民医院分院妇科,200081 [2]郑州大学公共卫生学院,450001 [3]河南省焦作市第二人民医院妇产科,454001

出　　处：《肿瘤研究与临床》2016年第1期15-20,共6页Cancer Research and Clinic

摘　　要：目的探索卵巢癌患者血清自体荧光光谱特征性差异的分子基础及手术治疗对光谱的影响。方法使用荧光分光光度计300nm激发光，检测84例卵巢癌患者手术前后及30名健康人血清自体荧光光谱，同时检测所有卵巢癌患者的血清肿瘤标志物及血红蛋白和血浆清蛋白水平，分析各指标与自体荧光光谱特征参数的相关性。结果与健康人相比，卵巢癌患者手术前血清白体荧光光谱在λ2峰位发生紫移，在λ4峰位发生红移；在λ1、λ2、λ4及λ6的峰强度（峰高）增高；在λ1-λ4及λ6峰位的峰面积增大。与手术前相比，卵巢癌患者术后血清自体荧光光谱在λ1～λ3峰位均发生红移，在λ1。λ4峰位的峰高降低，在λ1。λ4及λ6峰位的峰面积降低。卵巢癌患者血浆癌胚抗原（CEA）的水平与λ2峰高、λ2和λ3峰面积正相关；癌抗原125（CA125）水平与λ1～λ4及λ6的峰高以及λ1～λ3的峰面积正相关；CA199水平与λ2峰位负相关，与λ1～λ6的峰高及λ1一λ3、λ6的峰面积正相关；血浆清蛋白水平与λ2峰位正相关，与λ1-λ6的峰及λ1～λ3的峰面积负相关；血红蛋白水平仅与λ1峰位正相关。结论卵巢癌患者由于血清肿瘤标志物升高，清蛋白水平降低，导致其血清白体荧光光谱特征性参数发生改变。手术治疗可以部分恢复卵巢癌患者的血清白体荧光光谱改变。Objective To explore the molecular basis of the characteristics of the serum auto- fluorescent spectrum in patients with ovarian cancer and the changes that might be induced by surgery. Methods Using fluorospectrophotometer and 300nm excitation light,the serum auto-fluorescent spectrum of 84 patients with ovarian cancer before and after the surgery and 30 healthy people were detected. Meanwhile, the serum tumor signs （CEA, CA199 and CA125）, hemoglobin and plasma albumin level of all patients with ovarian cancer were detected. Their correlation with the fluorescence spectral characteristic parameters were analyzed. Results Compared with healthy people, the serum auto-fluorescent spectrum in patients with ovarian cancer exhibited purple-shifted position of λ2 peak and red shift in λ4 peak,had higher peak extent in kl, λ2, λ4 and k6 peak, and larger peak area of kl, λ2, λ3, λ4 and k6. Compared with those in ovarian cancer patients before surgery, the serum auto-fluorescent spectrum in these patients after operation had red shifts in kl, λ2 and λ3 peak, lower peak extent in λ1, λ2, λ3 and λ4, and smaller peak area in kl, λ2, λ3, λ4 and k6 peak. In ovarian cancer patients, the serum level of CEA was positively correlated with the λ2 peak extent and the peak area of λ2 and λ3, while the serum level of CA125 was positively correlated with the peak extent of kl-λ4 and k6 and the peak area of kl-λ3. The serum level of CA199 was negatively correlated with the h2 position and positively correlated with the peak extent of λ1-λ6 and the peak area of λ1-λ3 and λ6 in patents with ovarian cancer. Besides, the serum albumin was positively correlated with the λ2 peak position and negatively with the peak extent of λ1-λ6 and the λ1-h3 peak area, while the level of hemoglobin was positively correlated with λ1 peak position. Conclusions The elevated serum tumor markers and lower albumin （plasma） level lead to the changes of the serum autofluorescence spectra characteristic parametersin in patien

关 键 词：卵巢肿瘤 光谱法 荧光 血清 肿瘤标志 生物学 血浆清蛋白 血红蛋白 

分 类 号：R737.31[医药卫生—肿瘤]