四倍体青贮玉米染色体加倍及检测技术研究  

Research on Doubling Chromosome and Ploidy Identification of Silage Maize

作　　者：齐欣[1] 姜敏[1] 马骏[1] 刘欣芳[1] 弓雪[1] 李明[1] 王延波[1] 

机构地区：[1]辽宁省农业科学院玉米研究所,沈阳110161

出　　处：《玉米科学》2016年第1期28-32,共5页Journal of Maize Sciences

基　　金：辽宁省外专局项目(四倍体青贮玉米研发);辽宁省科技厅项目(2014201003;201501112);国家转基因重大专项(2014ZX08003-004;2014ZX08003-003);国家自然科学基金项目(31401392)

摘　　要：以辽单527、辽单青贮178、辽原一号3个玉米品种为试验材料,通过6个浓度的秋水仙素溶液处理,进行染色体加倍,采用流式细胞仪检测加倍效果。结果表明,当秋水仙素浓度达0.1%时,3个品种加倍率均达到最高值,辽单527的加倍效果最好,最高可达36.3%。利用流式细胞仪能够快速准确检测染色体倍性,OTTO法制备悬浮液的检测效果较好。In order to detect the double effect by flow cytometry（FCM）, there varieties of silage maize including Liaodan527, Liaodan178 and Liaoyuan No.1 were chosen as the materials, and 6 concentrations of colchicine were used for chromosome doubling. The results showed that, the doubling rate were all reached the highest level when the concentration of colchicine was 0.1%, and that of Liaodan527 was the best, which was 36.3%; FCM could cor- rectly detect the chromosome multiple and OTTO method had better effect.

关 键 词：玉米 秋水仙素 染色体检测 

分 类 号：S513.035.2[农业科学—作物学]