丁酸胁迫耦联丙酮丁醇梭菌-酿酒酵母混合培养强化丁醇发酵性能  

Enhanced ABE fermentation performance by co-culturing C. acetobutylicum /S. cerevisiae coupling with butyrate addition and stress

作　　者：何珍妮 盖来兵[2] 罗洪镇 张敬书[2] 赵艳丽 段作营[1] 史仲平[1] 

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]石家庄制药集团有限公司,河北石家庄050038 [3]河北常山生化制药股份有限公司,河北石家庄050800

出　　处：《工业微生物》2016年第1期1-7,共7页Industrial Microbiology

基　　金：国家自然科学基金(20976072)

摘　　要：为改善丁醇发酵性能,提出丁酸胁迫与丙酮丁醇梭菌-酿酒酵母混合培养体系协同作用的新型丁醇发酵优化控制策略。7 L发酵罐中,在溶剂生产期(24 h)添加4.0 g/L-broth的丁酸浓缩液和0.2 g-DCW/L-broth的酿酒酵母进行发酵,丁醇浓度、丁醇/丙酮比和总溶剂生产效率与对照相比分别提高35%、43%和79%,达到15.74 g/L、2.83和0.52 g/L/h的最高水平。若将精馏后溶剂混合物作为高效柴油添加剂,柴油添加剂中B∶A∶E比例可达74∶17∶9(w/w)的高水平,产品质量获得显著改善。试验及分析阐明该优化控制策略可大幅诱发赖氨酸的分泌及在梭菌中的吸收/利用,提高梭菌对高丁醇浓度环境的耐受能力,促进丁醇合成;可强化梭菌对底物利用的竞争能力、提高电子往复穿梭传递系统中还原力再生速率、产生更多用于丁醇合成的NADH。两者的协同作用大幅提高了丁醇发酵的整体性能。In order to enhance acetone-butanol-ethanol（ ABE） fermentation performance,a novel strategy of co-culturing C. acetobutylicum / S. cerevisiae coupling with exogenous butyrate addition and stress was proposed and conducted in a 7 L anaerobic bioreactor. During solventogenic phase of ABE fermentation by adding 0. 2 g-DCW / L-broth of S. cerevisiae and4. 0 g / L-broth concentrated butyrate solution into C. acetobutylicum culture broth in one time（ 24 h）, butanol concentration,butanol / acetone ratio and ABE productivity largely increased by 35%,43% and 79% as compared with those of the control,and reached the highest levels of 15. 74 g / L,2. 83 and 0. 52 g / L / h,respectively. If the purified ABE solvents mixture could be directly used as the diesel additive,the B∶ A∶ E ratio would vary and reach a higher level of 74∶ 17∶ 9（ w / w）,and the significant quality improvement of the additive product could be expected. Theoretical and experimental analysis revealed that the proposed strategy could extensively induce lysine secretion and its assimilation /utilization in C. acetobutylicum,promote C. acetobutylicum tolerant ability against high butanol environment and butanol synthesis; simultaneously enhance the competitive ability of C. acetobutylicum on substrate utilization and the reductive power originated from the electron transport shuttle system,to induce more intercellular NADH oriented for butanol synthesis in C. acetobutylicum metabolism. The synergetic actions of effective lysine assimilation and high NADH utilization rate desirable for butanol synthesis enhanced the ABE fermentation performance.

关 键 词：丁醇 丙酮丁醇梭菌 丁酸 赖氨酸 NADH 

分 类 号：TQ921.4[轻工技术与工程—发酵工程]