胆囊收缩素诱导的急性胰腺炎对小鼠自由节律的影响研究  

Study of the Effect of Cholecystokinin-Induced Acute Pancreatitis on the Free-Running Rhythm of Mouse

作　　者：李永红[1] 杨小平[1] 郭盼盼[1] 刘延友[2] 严洪立 李率真 关俊文[3] 

机构地区：[1]成都信息工程大学电子工程学院物理场生物效应及仪器四川高校重点实验室,成都610225 [2]四川大学卫生部时间生物学重点实验室,成都610041 [3]四川大学华西医院神经外科,成都610041

出　　处：《生物医学工程学杂志》2016年第1期115-119,125,共6页Journal of Biomedical Engineering

基　　金：四川省教育厅重点资助项目(2012ZA022);国家重点基础研究发展规划资助项目(2011CB711000);四川省科技创新苗子工程资助项目(2014Z057)

摘　　要：本文研究了胆囊收缩素(CCK)诱导的急性胰腺炎对ICR小鼠自发性活动自由节律的影响,分析生物节律与炎症性疾病及急性胰腺炎病理机制的相互关系。通过对全黑状态下的转轮甄别活动相和休息相行CCK诱导的急性胰腺炎造模,对比造模后的炎症程度以及两组前后周期、相位等节律指标的变化。结果发现相同剂量的急性胰腺炎ICR小鼠模型,休息相造模组比活动相造模组炎症程度重;且急性胰腺炎ICR小鼠活动节律的周期延长,在休息相造模组尤为明显。研究表明CCK诱导的急性胰腺炎可影响ICR小鼠的自由活动节律,且自由运转状态下不同相位造模时炎症轻重有明显区别,为急性胰腺炎重症化趋势的病理机制研究提供了可能的线索。The present paper reports the effect of pancreatitis induced by cholecystokinin（CCK）on free-running rhythm of locomotor activity of the ICR mice,and analyzes the interaction of inflammatory diseases and acute pancreatitis with circadian rhythm system.In the study,the mice were modeled under different phases of acute pancreatitis in DD status（Double Dark,constant dark condition）.By comparing of the inflammatory status and the indicators of rhythm before and after modeling of the running wheel activity group and the rest group,it was observed that the rest group showed more possibility of inflammation than the activity group did in ICR mice model of acute pancreatitis.In the rest phase model,the extension of the period is particularly longer.The results presented indicated that CCK-induced acute pancreatitis impacted free activity rhythm of ICR mice.Also in a free running model under different phase,the inflammation severity was proved significantly different.This study provides possible clues for the research of the pathogenesis of acute pancreatitis severe tendency.

关 键 词：胆囊收缩素 急性胰腺炎 生物节律 自由运转 

分 类 号：R576[医药卫生—消化系统]