Wnt2与Dvl蛋白在食管鳞癌组织中的表达及临床意义  

Expression and clinical significance of Wnt2 and Dvl protein in esophageal aquamous carcinoma

作　　者：王勇涛[1] 王洪江[1] 王兴名 冯雪[1] 

机构地区：[1]新疆医科大学附属肿瘤医院胸外科,830011

出　　处：《天津医药》2016年第2期210-212,共3页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(81160271)

摘　　要：目的检测Wnt2与散乱(Dvl)蛋白在食管鳞癌组织中的表达情况,探讨其与食管鳞癌发生发展的关系。方法采用Western blot法分别检测Wnt2、Dvl蛋白在60例食管鳞癌组织及其对应癌旁组织中的表达,并分析其与临床病理特征之间的关系。结果 Wnt2和Dvl蛋白在食管鳞癌组织中的相对表达量(0.512±0.406和1.218±1.082)高于癌旁食管组织(0.153±0.189和0.505±0.358),且Wnt2及Dvl蛋白表达在不同浸润深度、TNM分期及淋巴结转移情况中存在差异(P<0.01);Wnt2与Dvl蛋白在食管鳞癌组织中的表达呈正相关(r=0.718,P<0.01)。结论 Wnt2、Dvl蛋白的高表达通过Wnt2信号转导通路协同促进食管鳞癌的发生、发展及浸润转移。Objective To detect the expressions of Wnt2 and dishevelled （Dvl） protein in esophageal squamous carcinoma, and analyze their relationship with the occurrence and development of esophageal squamous cell carcinoma. Methods The expression levels of Wnt2 and Dvl protein were detected by Western blot assay in 60 samples of esophageal carcinoma and adjacent non-carcinomatous esophageal tissues, and their relationship with clinical pathological features were analyzed. Results The relative expression levels of Wnt2 and Dvl protein were higher in esophageal squamous carcinoma tissue （0.512 ± 0.406, 1.218±1.082） than those of esophageal tissue adjacent to carcinoma （0.153 ± 0.189, 0.505±0.358）. There were significant differences in the expression levels of Wnt2 and Dvl protein between different infiltration depth, different TNM stages, and lymph node metastasis （P 〈 0.01）. There was a positive correlation between Wnt2 and Dvl protein in esopha- geal squamous carcinoma （r = 0.718, P〈0.01）. Conclusion The high expression levels of Wnt2 and Dv1 protein promote the development and metastasis of esophageal squamous cell carcinomas collaboratively via Wnt2 signal transduction pathways.

关 键 词：食管肿瘤 癌 鳞状细胞 Wnt2蛋白质 Dvl蛋白质 印迹法 蛋白质 

分 类 号：R735.1[医药卫生—肿瘤]