镉对不同发育阶段中华稻蝗抗氧化性的影响  

Effects of Cd^(2+) on Anti-oxidative Capacity in Different Developing Stage of Oxya chinensis(Orthoptera Acridoidae)

作　　者：张晓明[1] 李丽君[1] 马恩波[2] 张建珍[2] 郭亚平[2,3] 

机构地区：[1]山西省农业科学院农业环境与资源研究所,山西太原030031 [2]山西大学生物应用学研究所,山西太原030006 [3]山西大学生命科学学院,山西太原030006

出　　处：《山西农业科学》2016年第2期152-157,共6页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31071980);高等学校博士点基金项目(20111401110006);山西省回国留学人员科研资助项目(2008114);山西省农业科学院博士基金项目(YBSJJ0702)

摘　　要：采用急性染毒方法研究Cd^(2+)胁迫对不同发育阶段中华稻蝗胸部、腹部谷胱甘肽过氧化物酶(GPx)活性、谷胱甘肽硫转移酶(GST)活性和总抗氧化能力(T-AOC)的影响。结果表明,不同质量浓度的Cd^(2+)作用于不同发育阶段中华稻蝗体内时,其胸部和腹部的GPx,GST活性及T-AOC对Cd^(2+)胁迫的应激反应程度不同。说明Cd^(2+)可影响中华稻蝗的GPx,GST活性及T-AOC,中华稻蝗对Cd^(2+)有一定的耐受性,且这种耐受性与中华稻蝗的龄期及性别有关。中华稻蝗在受到Cd^(2+)胁迫时将调动其体内的抗氧化机制,维持H_2O_2产生与清除的平衡,有效地清除过量的ROS,保护机体免受外界不利因素的损害,进而避免机体DNA、蛋白质等功能的损伤。中华稻蝗可作为农业环境中Cd^(2+)的指示生物。To explore and clarify the responses of Oxya chinensis to Cd^（2＋） stress, provide the scientific evaluation of Cd^（2＋） tolerance,the toxic effect of Cd^（2＋） on activities of glutathione peroxidase（GPx）and glutathione S-transferase（GST）, and the total anti-oxidative capacity（T-AOC）in different developing stage of O. chinensis injected with Cd^（2＋） were determined. Different developing stages O.chinensis were injected with Cd^（2＋） at different concentrations（0, 50, 100, 150, 200 mg/L）, and they were collected after 24 h. The results showed that the effect of Cd^（2＋） on GPx and GST activities and T-AOC were different within the experimental gradient. The GPx and GST activities, and T-AOC depended on the content of oxygen species（ROS）under Cd^（2＋） stress in the experiment, and Cd^（2＋） could induce damage of antioxidant defense system in O. chinensis, but O. chinensis kept its GPx and GST activities, and T-AOC increasing from Cd^（2＋） damage in some Cd^（2＋） contents. The tolerance of Cd^（2＋） changed in different developing stages and male or female O. chinensis. Variety of GPx and GST activities, and T-AOC in different developing stages and male or female O. chinensis might act as indicative functions for Cd^（2＋）contamination.

关 键 词：中华稻蝗 镉 总抗氧化能力 谷胱甘肽过氧化物酶 谷胱甘肽硫转移酶 

分 类 号：X174[环境科学与工程—环境科学]