肝豆灵对铜负荷大鼠肝细胞Bax、Bcl-2表达的影响  

Infl uences of Gandouling on the Expressions of Bax and Bcl-2 in Hepatocytes of Copper loading Rats

作　　者：蒋怀周[1] 吴生兵[1] 沈斌[2] 刘磊[3] 

机构地区：[1]安徽中医药大学,安徽合肥230031 [2]泰州市中医院,江苏泰州225300 [3]亳州市人民医院,安徽亳州236800

出　　处：《中医药临床杂志》2016年第1期94-97,共4页Clinical Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金(81202691);安徽省高校自然科学基金重点项目(KJ2012Z228);安徽中医学院自然科学基金(2011ZR008B);安徽省高校博士后岗位项目基金

摘　　要：目的:观测肝豆灵对铜负荷大鼠肝细胞Bax和Bcl-2表达的影响,分析该方对铜负荷大鼠肝损伤的治疗作用机制。方法:采用铜负荷法制作大鼠模型,造模和药物干预结束后,以免疫组化技术测大鼠肝细胞Bax和Bcl-2表达。结果:与对照组相比,模型组肝细胞Bax和Bcl-2表达升高(P<0.05)。与模型组相比,肝豆灵组肝细胞Bax表达降低(P<0.05),Bcl-2表达升高(P<0.05)。结论:肝豆灵对铜负荷大鼠肝损伤具有一定的干预作用,机制可能与下调Bax,上调Bcl-2的表达有关。Objective： To explore the influences of Gandouling on the expressions of Bax and Bcl-2 in hepatocytes of copper-laden rats, so as to analyze its treatment mechanism on rats with liver injury caused by copper-laden. Methods： Copper-laden method was used to establish model rat. Immunohistochemistry was used to detect expressions of Bax and Bcl-2 in liver cells. Results： Compared with the control group, Bax and Bcl-2 expressions were increased （P〈0.05）.Compared with the model group, the Gandouling group had lower Bax expression （P〈0.05）, and higher Bcl-2 expression （P〈0.05）. Conclusion： Gandouling has a certain therapeutic effect on rats with liver injury caused by copper-laden. Its mechanism may be related to down-regulation of Bax and up-regulation of Bcl-2.

关 键 词：肝细胞凋亡 肝豆灵 铜负荷 

分 类 号：R741.05[医药卫生—神经病学与精神病学] R595.2[医药卫生—临床医学]