广东潮汕贲门癌组织中GST-Π和P-gp的表达及其临床病理意义  

Expression of GST-π and P-gp in gastric cardia cancer and their clinical significance in the Chaoshan high risk area

作　　者：陈广灿[1] 洪良利[2] 黄杰雄[2] 谢澳斯[1] 郭丹[3] 刘淑慧[3] 

机构地区：[1]汕头大学医学院第一附属医院胃肠外科,广东汕头515041 [2]汕头大学医学院第一附属医院病理科,广东汕头515041 [3]汕头大学医学院病理教研室,广东汕头515041

出　　处：《癌变．畸变．突变》2016年第1期36-40,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：广东省自然科学基金资助(2014A030310139)

摘　　要：目的:探讨广东潮汕贲门癌患者癌组织中谷胱甘肽-巯基-转移酶-π(GST-π)和P-糖蛋白(P-gp)的表达情况及二者与临床病理指标的关系。方法:采用免疫组织化学法检测87例贲门癌组织GST-π和P-gp的表达情况。根据表达情况,利用Youden index法绘制患者的年龄和肿瘤最长径ROC曲线,并将患者分成高表达组和低表达组比较其与临床病理指标的关系。结果:贲门癌组织中GST-π和P-gp表达阳性率分别为85.05%和93.1%。根据ROC曲线的结果确定年龄和肿瘤最长径分组的界值。贲门癌GST-π表达与脉管是否浸润有关,有脉管浸润者高表达率明显高于无脉管浸润者(P<0.01);P-gp的表达与年龄和组织学类型相关,患者年龄增大表达率增高(P<0.05),黏液腺癌的高表达率明显高于非黏液腺癌(P<0.05)。贲门癌GST-π的表达与P-gp的表达呈正相关(r=-0.828,P<0.01)。结论:GST-π和P-gp在潮汕贲门癌组织中阳性表达率高,两者的表达呈正相关。GST-π高表达与脉管浸润相关,P-gp高表达与高龄患者和贲门黏液腺癌相关。OBJECTIVE：To study the expression of glutathione-s-transferase-π（GST-π） and P-glycoprotein（P-gp）in gastric cardia the cancer in the Chaoshan high risk area and their relationships with clinical pathological characteristics of gastric cardia cancer.METHODS：Immunohistochemical staining was used to detect the expression of GST-π and P-gp in87 cases of gastric cardia cancer.The correlations between GST-π,P-gp expression and the clinical characteristics of gastric cardia cancer were analyzed.ROC curves of age and maximum tumor diameter were plotted.RESULTS：The positive expression rate of GST-π and P-gp in gastric cardia cancer were 85.05%and 93.1%,respectively.The cutoff points of grouping for age and maximum tumor diameter depended upon the results of ROC curves.The expression of GST-re was related to lymph-vascular space invasion.The expression rate of GST-π of patients with lymph-vascular space invasion was significantly higher than that without the invasion（P〈0.05）.Expression of P-gp was related to the age of patients and the histological type of gastric cardia cancer.Cancer tissues from elder patients and mucinous adenocarcinomas showed significantly higher rate of expression of P-gp（P〈0.05）.GST-π expression was positively correlated to P-gp expression（P〈0.01）.CONCLUSION：GST-π and P-gp expression were high in gastric cardia cancer in the Chaoshan area.Their expression was positively correlated.High GST-π expression was associated with lymph-vascular space invasion.High P-gp expression was associated with elder patients and mucinous adenocarcinoma tissues.

关 键 词：贲门癌 谷胱甘肽-巯基-转移酶-π P-糖蛋白 免疫组织化学 

分 类 号：R730.2[医药卫生—肿瘤]