球形红细菌厌氧降解2,4-二硝基甲苯（英文）  

Anaerobic biodegradation of 2,4-dinitrotoluene by Rhodobacter sphaeroides

作　　者：梁芳楠 白红娟[1] 柴春镜[2] 王姗[3] 牛伟平 赵婷婷[1] 

机构地区：[1]中北大学化工与环境学院,山西太原030051 [2]中北大学朔州校区化工与环境学院,山西朔州036000 [3]北京航天计量测试技术研究所,北京100076 [4]山西省农药检定所,山西太原030001

出　　处：《微生物学通报》2016年第2期279-284,共6页Microbiology China

基　　金：Enterprise Program Foundation of Shanxi Province(No.20080311027-1)

摘　　要：【目的】研究不同环境条件对2,4-二硝基甲苯(2,4-DNT)生物降解的影响。【方法】采用光合细菌球形红细菌在温度为30°C的光照培养箱中厌氧降解2,4-DNT,并用高效液相色谱仪测定其浓度。【结果】去除2,4-DNT的最佳条件是初始浓度40 mg/L、初始p H 7.0和接种量15%。另外,2,4-DNT在菌体延滞期被细胞吸收,然后在指数期作为碳源被降解。2,4-DNT的去除率在72 h达到98.8%。从液相色谱图中观察到有2种中间代谢产物,但在120 h内产物被逐渐降解。2,4-DNT的去除动力学符合一级速率模型。【结论】不同条件下2,4-DNT的去除率表明球形红细菌能有效降解2,4-DNT。[Objective] To investigate the biodegradation of 2,4-dinitrotoluene（2,4-DNT） under various environmental conditions by Rhodobacter sphaeroides. [Methods] 2,4-DNT was anaerobically biodegraded under 30 ℃ in illumination incubator using photosynthetic bacterium Rhodobacter sphaeroides. The concentration of 2,4-DNT in liquid medium were detected by HPLC. [Results] Optimum conditions for the removal of 2,4-DNT were initial concentration of 40 mg/L, initial p H 7.0 and inoculation quantity of 15%. In addition, 2,4-DNT could be absorbed by the cells in lag phase, then degraded as carbon source in exponential phase. The removal rate of 2,4-DNT achieved 98.8% at 72 h. Using HPLC, two different intermediate metabolites were also observed, however, these decreased gradually within 120 h. Furthermore, the removal kinetics of 2,4-DNT corresponded with the first-order rate model. [Conclusion] The removal rate of 2,4-DNT under different conditions indicated that Rhodobacter sphaeroides is efficient in biodegrading 2,4-DNT.

关 键 词：厌氧降解 2 4-DNT 动力学 球形红细菌 

分 类 号：X172[环境科学与工程—环境科学]