基于肺胃P物质表达对中医肺胃相关理论内在机制的研究  

Study of Internal Mechanism of Lung and Stomach Theory Based on Expression of Substance P

作　　者：何飞[1] 徐俭朴[1] 杨明华[2] 周勇[1] 洪辉华[3] 

机构地区：[1]浙江中医药大学附属中西医结合医院,浙江杭州310003 [2]浙江省中药研究所,浙江杭州310023 [3]浙江中医药大学附属第一医院,浙江杭州310006

出　　处：《中华中医药学刊》2016年第2期315-317,I0005,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81403336);浙江省中医药科技计划项目(2011ZB108)

摘　　要：目的:观察慢性食管内灌注盐酸是否可以诱导大鼠迷走神经相关的食管-支气管反射通路神经肽P物质表达发生改变,并进一步探索中医肺胃相关理论的物质基础。方法:将16只雄性SD大鼠,按随机数字表法分正常对照组(n=8)、盐酸组(n=8),盐酸组用戊巴比妥钠腹腔注射轻度麻醉大鼠,将6号胃管插入食管至食管中下端,灌注含0.5%胃蛋白酶的0.1 mol/L盐酸(8滴/min,20 min/d),连续灌注14 d。正常对照组用PBs代替盐酸灌注食管,提取食管、气管和肺组织标本进行病理学观察和P物质免疫组化法测定。结果:盐酸组与正常对照组相比,食道、胃及肺组织中P物质表达均明显增多(P<0.01)。结论:慢性食管内灌注盐酸可以诱导大鼠产生气道神经源性炎症,及食管-支气管反射通路发生P物质表达改变,提示P物质可能是中医肺胃相关理论的物质基础之一。Objective： To investigate if repeated intra- esophageal instillation of hydrochloric acid（ HCl） may induce the change of neuropeptide in esophageal-bronchil reflex pathway associated with vagus nerve in rats and to explore the material basis of lung and stomach theory in traditional Chinese medcine. Methods： 24 male SD rats were randomized into2 groups： bland control group and HCl group.Animals in HCl group received instillation of 0.1 mol / L of HCl containing 0.5% pepsin at middle and lower parts of esophagus once a day for 14 days. The expressions of substance P（ SP） in the airway,esophagus and Stomach were detected by immunohistochemistry. Results： The expressions of SP in the airway,esophagus and stomach in HCI group were significantly higher than those in bland control group（ P〈0.05 and P〈0.01）. Conclusion： Repeated intra-esophageal instillation of HCl can induce airway neurogenic inflammation and SP alternations in esophageal-bronchial reflex pathway in rats,which indicated that SP would be the material basis of lung and stomach theory in traditional Chinese medcine.

关 键 词：P物质 肺胃相关 食管-支气管反射通路 

分 类 号：R-332[医药卫生]