高效苯胺降解菌的分离鉴定及其降解特性  

Isolation and Identification of Bacteria Degrading Aniline

作　　者：吕晶华[1] 段云霞[2] 许丹宇[2] 郑先强[2] 石岩[1] 张金鸿[2] 

机构地区：[1]天津市联合环保工程设计有限公司,天津300191 [2]天津市环境保护科学研究院,天津300191

出　　处：《城市环境与城市生态》2016年第1期32-34,共3页Urban Environment & Urban Ecology

基　　金：国家自然科学基金(51178311);天津市科技计划项目(13ZCZDSF00700)

摘　　要：使用富集、稀释涂平板的方法从处理染料废水的活性污泥中筛选到6株苯胺降解菌,经16S r DNA鉴定为Delftia tsuruhatensis、Delftia sp.、Uncultured Delftia sp.、Delftia sp.、Acinetobacter sp.和Delftia tsuruhatensis。对各单一菌种生长条件的研究表明,各菌种生长的最适p H为7,最适生长温度为37℃。在最适生长条件下,菌种BN6对苯胺降解效果最佳,在48 h内可将2 000 mg/L的苯胺完全降解且可耐受的苯胺浓度最高,为5 500 mg/L。Six bacteria were screened with the method of enrichment and dilution from the sludge to treat dye wastewater. The six bacteria were identified as Delftia tsuruhatensis, Delftia sp., Uncultured Delftia sp., Delftia sp.,Acinetobacter sp., and Delftia tsuruhatensis by 16 S r DNA identification. The study on the growth conditions of the six bacteria showed that the optimal p H was 7 and the optimal growth temperature was 37℃. The study on degradation of aniline by the single bacterium was carried under the optimal growth conditions. The results showed that degradation efficiency of Delftia tsuruhatensis BN6 was better than other single bacteria and it could degrade aniline about 2 000mg/L in 48 hours. The BN6 could toterate aniline at a concentration of 5 500 mg/L.

关 键 词：菌种筛选 16S rDNA鉴定 苯胺降解 

分 类 号：X703.1[环境科学与工程—环境工程]