大孔树脂对核桃青皮中胡桃醌的分离纯化  

Separation and purification of juglone from walnut green husk by macroporous resin

作　　者：马乐[1] 张有林[1] 韩军岐[1] 周星余[1] 夏莎莎[1] 

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710119

出　　处：《食品与发酵工业》2016年第1期108-113,共6页Food and Fermentation Industries

基　　金：西安市农业公关项目(NC1505.4)

摘　　要：比较6种不同极性大孔树脂对核桃青皮提取物中胡桃醌的静态吸附和解吸性能,筛选出最佳的大孔树脂。通过与胡桃醌标准品HPLC保留时间相比较的方法对胡桃醌含量进行分析。结果表明:D101树脂为纯化核桃青皮胡桃醌的最佳树脂,较佳的动态吸附条件为上样液胡桃醌浓度0.114 mg/m L,p H 2.0,上样速率为1.0 m L/min,吸附率可达81.85%;较佳的洗脱条件为体积分数95%的丙酮,p H 4.0,洗脱速率为0.33 m L/min,解吸率为76.5%。经HPLC鉴定和分析,D101分离纯化核桃青皮胡桃醌不会造成组成变化和较大损失。D101大孔树脂对核桃青皮胡桃醌有较好的分离纯化效果。The content of juglone from walnut green husk extraction was relatively low,which influences its biological activity. So it is important to find a simple and effective method to separate and purify it. Moreover,the optimum technology and conditions were also needed. Method： The static adsorption and desorption performance of six different polarities macroporous resins to juglone of walnut green husk extracts were compared to select the most suitable resin. The juglone content of walnut green husk extracts were analyzed by HPLC（ high performance liquid chromatography） through comparing to the retention time of juglone standard. Result： Macroporous resin D101 was the best one for purification of walnut green husk juglone. Its optimum absorption condition was： feeding concentration0. 114 mg / m L,p H2. 0,feeding rate 1. 0m L / min,then the adsorption ratio was 81. 85%. The optimum desorption conditions were： elute acetone concentration 95%,p H4. 0,elution rate 0. 33 m L / min. The desorption ratio could reach to 76. 5%. Identified and analyzed by HPLC,there was no large lose and change on the juglone profiles of walnut green husk with D101 macroporous resin purification. Conclusion： D101 macroporous resin could be used to purify juglone of walnut green husk.

关 键 词：核桃青皮 胡桃醌 大孔树脂 分离纯化 

分 类 号：TQ914.1[化学工程]