不同前处理对植物酯酶抑制法检测蔬菜中有机磷及氨基甲酸酯类农药残留的影响  

Effect of different pre-treatment methods on detection results of organophosphate and carbamate pesticide residues in vegetables by plant esterase inhibition method

作　　者：姜露[1] 叶麟[1] 杨雪[1] 黎杉珊[1] 申光辉[1] 张志清[1] 

机构地区：[1]四川农业大学食品学院,四川雅安625014

出　　处：《食品与发酵工业》2016年第1期200-204,共5页Food and Fermentation Industries

摘　　要：利用酶抑制法可以快速检测蔬菜中的有机磷和氨基甲酸酯类农药残留,这两类农药能抑制酯酶的活性,并影响酯酶对底物的催化能力。根据这一原理,采用麦麸酯酶作为酶源对酶抑制法快速检测农残前处理方法中农残提取液、提取方法、提取时间及样品提取液的处理方式进行优化。结果表明：用5%丙酮-磷酸盐缓冲液作为提取液,然后超声提取5 min后,将样品提取液再用0.45μm有机滤膜过滤后蔬菜中农药残留提取量可达到最高;且样品加标回收率为83.61%~102.81%,变异系数为1.20%~7.23%。因此,该前处理方法可满足于麦麸酯酶抑制法快速检测农药残留的需要。The activity of esterase can be inhibited by organophosphate and carbamate pesticide residues. The ability of the esterase to catalyze the substrate can also be reduced. Using this principle,the optimizations of pesticide residue extraction solution,extracting methods,extraction time and the treatment of sample solution were separately done by using wheat bran enzyme inhibition method. The results showed that the optimal way to extract pesticides residues in vegetables was 5% acetone / PBS extracting 5 min by ultrasonic,then filtered with 0. 45 μm organic film.With the above pre-treatment methods,the response rate ranged from 83. 61% to 102. 81%,with coefficient of variation at the ranged from 1. 2% to 7. 23%. Therefore,this pre-treatment can meet the need of rapid detection of pesticide residues.

关 键 词：麦麸酯酶 酶抑制法 有机磷农药 氨基甲酸酯类农药 蔬菜 前处理方法 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]