组织蛋白酶B在膀胱移形细胞癌中表达与复发的相关性  

Expression of cathepsin B in bladder transitional cell carcinoma tissues and its correlation with the recurrence of carcinoma

作　　者：唐硕[1] 魏强[2] 王安果[1] 张宗平[1] 文焰林[1] 李云祥[1] 

机构地区：[1]川北医学院第二临床医学院·南充市中心医院泌尿外科,四川南充637000 [2]四川大学华西医院泌尿外科,四川成都610041

出　　处：《西部医学》2016年第2期166-168,共3页Medical Journal of West China

基　　金：南充市科技局资助项目(2010SF13)

摘　　要：目的 探讨组织蛋白酶B（CB）在膀胱移行细胞癌（BTCC）组织中的表达及与肿瘤复发的关系.方法 收集1994～2011年复发的膀胱移形细胞癌标本,共计病理组织切片172份,用链霉素抗生物素蛋白-过氧化物酶连接法行CB免疫组织化学染色并计算CB阳性细胞百分率.结果 癌组织中CB阳性细胞以弥散分布为主,细胞周围基质染色明显.BTCC组织中CB标记指数（21.53±4.27）较正常组织（0.34±0.18）明显升高（P＜0.05）;高分级癌组织中CB标记指数（27.25±4.82）较低分级（14.38±5.83）明显增高（P＜0.05）;复发组随复发次数进一步增高（P＜0.05）;分期高的癌组织中CB值（24.68士5.42）较低期癌组织中（17.24±6.23）明显升高（P＜0.05）.结论 组织蛋白酶B在正常膀胱粘膜中几乎不染色或仅见少许零星的阳性细胞,在分级和分期高的癌组织中染色明显,多为弥散阳性染色,CB可作为判断BTCC患者的进展和预后的一个重要指标.Objective To evaluation the expression of cathepsin B in the transitional cell carcinorma of bladder （BTCC） and its correlation with the recurrence of carcinoma. Methods 172 tissues were collected from BTCC patients recurred for 3 times or more （3 times is contained） from 1994 to 2010. Immunohistochemical staining of CB in TCC and normal tissues was carried out by the streptavidin-biotin method. Results Immunohistochemistry stain demonstrated that the positive cells of cathepsin B was diffused in the cancer tissues with obvious stroma staining. The label index of BTCC tissues was obviously higher than that of normal tissues（P〈0.01）, and the label index is increased with the recurrence time（P〈0. 01）. Conclusion The label index of high differentiation carcinoma is higher than that of low differentiation carcinoma. Cathepsin B staining of the normal bladder tissue is negative or only a few positive cells,but in the high grade and stage careimoma tissues. The staining is positive and dispersed. Cathepsin B should be a good tool to determine the bladder tumor behavior and its prognosis.

关 键 词：膀胱移形细胞癌 复发 免疫组化 

分 类 号：R737.14[医药卫生—肿瘤]