miR-21调控PTEN/Akt信号通路抑制肝癌HepG2细胞增殖的实验研究  

miR-21 regulates tumor cells proliferation through PTEN/Akt signaling pathway in hepatocellular carcinoma HepG2 cells

作　　者：王涛[1] 温桂海[1] 张桂东[1] 李恩君[1] 武向鹏[1] 苑昭奖 朱涛[2] 

机构地区：[1]邯郸市中心医院普外一科,河北邯郸056001 [2]四川大学华西医院,成都四川610041

出　　处：《西部医学》2016年第2期169-172,共4页Medical Journal of West China

基　　金：中国博士后科学基金(2014M552369)

摘　　要：目的探讨miR-21对于肝癌HepG2细胞增殖的调控作用及其相关的信号通路。方法分别采用miR-21mimic和miR-21inhibitor上调和下调肝癌HepG2细胞中miR-21的表达量。在不同时间点(0h、24h和48h)对肝癌HepG2细胞的增殖水平使用MTT法进行检测。在干预4h后,使用qPCR法对细胞中miR-21和PTEN mRNA水平进行检测;并对PTEN和Akt蛋白水平及Akt磷酸化(phospho-Akt)水平使用western blot进行检测。结果与对照组相比较,给予miR-21mimic干预的HepG2细胞活性呈时间依赖性增加,差异均有统计学意义(P均<0.05)。与对照组相比较,给予miR-21inhibitor干预的HepG2细胞活性呈时间依赖性降低,差异均有统计学意义(P均<0.05)。在干预4h后与对照组相比较,miR-21mimic干预的HepG2细胞miR-21mRNA和Akt磷酸化水平明显增加而PTEN mRNA和蛋白表达水平明显降低,差异均有统计学意义(P均<0.05);同时与对照组相比较,miR-21inhibitor干预的HepG2细胞miR-21mRNA和Akt磷酸化水平明显降低而PTEN mRNA和蛋白表达水平明显增加,差异均有统计学意义(P均<0.05)。结论该基础研究表明miR-21可能可以通过PTEN/Akt信号通路对肝癌HepG2细胞的增殖产生关键性的调控作用。并通过抑制miR-21的表达具有抗肝脏肿瘤的价值,为进一步肿瘤临床治疗提供思路。Objective To explore the value of miR-21 on the regulation of hepatocellular carcinoma HepG2 cells proliferation and its signaling pathway. Methods miR-21 mimic and miR-21 inhibitor were used to up-regulate and down- regulate the expression of miR-21 in hepatocellular carcinoma HepG2 cells. Then, MTT was performed to evaluate HepG2 ceils viabilities. And qPCR was included to analyze the mRNA expression of miR-21 and PTEN. Meanwhile, the protein expression of PTEN and the phosphorylation of Akt were detected by western blot. Results Our data showed that the cell viabilities of HepG2 cells were time-dependently increased by miR-21 mimic, and, time-dependently de- creased by miR-21 inhibitor. And after 4h of interventions, miR-21 mimic up-regulated and miR-21 inhibitor down-regu- lated the mRNA of miR-21 and the phosphorylation of Akt, respectively. Meanwhile, the mRNA and protein expression of PTEN was inhibited by miR-21 mimic and promoted by miR-21 inhibitor. Conclusion These resuhs indicate that miR- 21 could mediate the tumor proliferation possibly through PTEN/Akt signaling pathway in hepatocellular carcinoma HepG2 cells.

关 键 词：MIR-21 PTEN AKT HEPG2细胞 

分 类 号：R735.7[医药卫生—肿瘤]