马齿苋多糖对宫颈癌细胞恶性生物学行为抑制作用的实验研究  

Animal study on the inhibition effect of Portulaca oleracea polysaccharide on malignant behavior of cervical cancer cells

作　　者：丁虹[1] 唐雯静[1] 庞燕芬[1] 吕雯 

机构地区：[1]南京军区杭州疗养院海勤疗养区妇科,浙江杭州310002 [2]浙江省立同德医院妇产科,浙江杭州310013

出　　处：《东南国防医药》2016年第1期38-40,58,共4页Military Medical Journal of Southeast China

摘　　要：目的研究马齿苋多糖对宫颈癌细胞恶性生物学行为的抑制作用。方法选择c57小鼠并建立宫颈癌荷瘤鼠模型,随机分为对照组、低剂量组、中剂量组和高剂量组4组,分别接受等渗盐水、100、200、400 mg/kg马齿苋多糖腹腔注射。治疗后检测移植瘤的体积、重量以及移植瘤中细胞凋亡数目、ATP含量、新生血管分子的表达量。结果低剂量组、中剂量组和高剂量组的瘤体体积和重量、移植瘤组织中的ATP含量以及血管内皮生长因子(VEGF)、CD13、CD34的表达量均低于对照组,凋亡细胞数目多于对照组。马齿苋多糖能够以剂量依赖性的方式降低移植瘤的体积、重量以及移植瘤中细胞凋亡数目、ATP含量、新生血管血管分子的表达量,增加凋亡细胞的数目。结论马齿苋多糖能够抑制宫颈癌移植瘤的生长、诱导癌细胞凋亡、干扰氧化供能、减少新生血管数目,对宫颈癌细胞恶性生物学行为具有抑制作用。Objective To study the inhibition effect of portulacaoleracea polysaccharide on malignant behavior of cervical cancer cells. Methods c57 mice were selected and cervical cancer bearing mice model were established and randomly divided into control group,low dose group,middle dose group and high dose group,which received saline,100 mg / kg polysaccharide,200 mg / kg polysaccharide,400 mg / kg polysaccharide injection respectively. After treatment,tumor volume,weight and apoptosis cell number,ATP content,neovascularization vascular molecules in tumor were detected. Results Tumor volume,weight,ATP content and VEGF,CD13,CD34 expression level in tumor of low dose group,middle dose group and high dose group were lower than control group; portulacaoleracea polysaccharide reduced tumor volume,weight,ATP content and VEGF,CD13,CD34 expression level in tumor by a dose-dependent manner. Conclusion Portulacaoleracea polysaccharide can inhibit cancer tumor growth,increase cell apoptosis number,induce apoptosis,interfere oxidation energy,reduce new blood vessels number and inhibit malignant behavior of cervical cancer cells.

关 键 词：宫颈癌 马齿苋多糖 凋亡 血管新生 三磷酸腺苷 

分 类 号：R737.33[医药卫生—肿瘤] R285.5[医药卫生—临床医学]