外源谷胱甘肽对干旱胁迫下玉米幼苗叶片生理特性的影响  

Effects of Exogenous Glutathione on Leaf Physiological Properties of Maize Seedling under Drought Stress

作　　者：单长卷[1,2] 代海芳[1,2] 

机构地区：[1]河南科技学院,河南新乡453003 [2]现代生物育种河南省协同创新中心,河南新乡453003

出　　处：《灌溉排水学报》2016年第1期59-62,共4页Journal of Irrigation and Drainage

基　　金：河南省教育厅科学技术研究重点项目(13A180302)

摘　　要：采用溶液培养的方法,研究了外源谷胱甘肽(GSH)对干旱胁迫下玉米幼苗叶片生理特性的影响。结果表明,干旱胁迫显著提高了叶片细胞质膜透性、MDA、SOD活性、APX活性、GSH、AsA、可溶性糖、脯氨酸和可溶性蛋白,显著降低了POD活性、CAT活性、叶绿素、类胡萝卜素、光合速率和单株生物量。外源GSH处理则可以显著提高干旱胁迫下叶片POD活性、SOD活性、APX活性、GSH、可溶性糖、脯氨酸、叶绿素、类胡萝卜素、光合速率及单株生物量,并显著降低了细胞质膜透性和MDA。上述研究结果说明,干旱胁迫对玉米造成了氧化胁迫和渗透胁迫,外源GSH处理则可以通过增强抗氧化酶活性、提高抗氧化物质和渗透调节物质而缓解干旱造成的伤害。To provide evidence for the application of exogenous glutathione（GSH）in water management of maize production and cultivation,the effects of exogenous glutathione on leaf physiological properties of Baiyu1 seedings under drought stress were investigated.The results showed that the membrane permeability,MDA content,the activities of SOD and APX,the contents of GSH,AsA,soluble sugar,proline and soluble protein in leaves were significantly improved by drought stress.However,the activities of POD and CAT,the contents of chlorophyll and carotenoid,photosynthetic rate and biomass per plant were significantly reduced by drought stress.The activities of POD,SOD and APX,GSH content,the contents of soluble sugar and praline,the contents of chlorophyll and carotenoid,photosynthetic rate and biomass per plant were significantly improved by exogenous GSH treatment.Besides,MDA content and the membrane permeability of leaves were significantly reduced by exogenous GSH.Our results indicated that oxidative stress and osmotic stress were induced by drought stress to maize seedlings.Exogenous GSH could enhance the activities of antioxidant enzymes,and increase the contents of antioxidant substances and osmotic adjustment substances under drought stress.Therefore,the injuries of maize induced by drought stress could be alleviated by exogenous GSH.

关 键 词：玉米 抗氧化特性 谷胱甘肽 干旱胁迫 渗透调节 

分 类 号：S513[农业科学—作物学]