蜗牛酶辅助降解茯苓多糖工艺优化研究  

Research on the Optimization of Auxiliary Extraction Process in Poria cocos Polysaccharides by Snailase

作　　者：李悦[1] 吴和珍[1,2] 叶丛进[1] 覃祝 王业静[1] 李群[1] 杨艳芳[1,2] 

机构地区：[1]湖北中医药大学,湖北武汉430065 [2]湖北中医药大学中药资源与中药复方教育部重点实验室,湖北武汉430065

出　　处：《亚太传统医药》2016年第4期53-55,共3页Asia-Pacific Traditional Medicine

摘　　要：目的:优选蜗牛酶降解茯苓多糖的反应条件。方法:以茯苓多糖酶解得率为指标,采用单因素试验针对影响茯苓酶解的pH、加酶率、固液比、反应温度、反应时间等5个主要因素分别加以研究,并利用正交试验进行优化。结果:优选茯苓多糖的降解条件为:pH=6,加酶率1.8%,固液比1∶20,酶解温度40℃,酶解时间5h。结论:在该优选条件下,茯苓多糖降解率为40.2%,证实蜗牛酶可有效降解茯苓多糖,提高茯苓多糖的水溶性,与传统的水提醇沉法比较,得率提高65%以上。Objective：In order to explore the optimal condition of Poria enzymatic hydrolysis,Methods：Snailase has been used through single-factor study and orthogonal experiment.The single-factors investigated includes：pH,enzyme rate,solid-liquid ratio,temperature and reaction time.With single-factor test results comprehensively considered,using L18（35）orthogonal arrangement test.Results：the optimum hydrolysis condition for Poria enzymatic hydrolysis：pH=6,1.8% of enzyme liquid ratio of 1：20,40℃as reaction temperature and 5hours as hydrolysis time,under which the yield of polysaccharides is 40.2%.Conclusion：it can effectively improve the water soluble of Poria cocos polysaccharides.Compared with the traditional Ethanol subsiding method,the yield increased by more than 65%.

关 键 词：蜗牛酶 茯苓多糖 降解工艺 

分 类 号：R286[医药卫生—中药学]