β-榄香烯对人食管癌细胞EC9706增殖、凋亡及caspase-3的影响  

Effect of β-elemene on Proliferation,Apoptosis and Activity of Caspase-3 of the Human Esophageal Carcinoma Cell EC9706

作　　者：任凌燕[1] 徐磊[1] 赵青[1] 罗皓[1] 赵怡[1] 

机构地区：[1]解放军第四五四医院,210000

出　　处：《实用癌症杂志》2016年第2期202-204,209,共4页The Practical Journal of Cancer

摘　　要：目的研究β-榄香烯对人食管癌细胞EC9706增殖、凋亡及caspase-3表达的影响。方法采用不同浓度的β-榄香烯(0、10、20、30、40、50μg/ml)处理细胞24 h,CCK-8测定细胞增殖活力。以50μg/mlβ-榄香烯处理细胞0、6、12、24 h,CCK-8测定细胞增殖活力。以不加β-榄香烯组作为对照组,选取50μg/mlβ-榄香烯处理细胞24 h,流式细胞术测定细胞凋亡率,分光光度法测定caspase-3活性。结果随着β-榄香烯浓度的增加,人食管癌细胞增殖活力逐渐下降,呈浓度依赖性(P<0.05)。以50μg/mlβ-榄香烯处理细胞0、6、12、24 h,随着时间的增加,细胞增殖活力下降,呈现时间依赖性(P<0.05)。相比对照组,50μg/mlβ-榄香烯处理细胞24 h后细胞凋亡明显增加(P<0.05),caspase-3活性明显增加(P<0.05)。结论β-榄香烯可抑制食管癌细胞增殖、诱导其凋亡,并且其诱导凋亡的作用机制可能与促进caspase-3活化有关。Objective To investigate the effect of β-elemene on the proliferation and apoptosis of human esophageal carcinoma cell EC9706,and to explore the possible mechanisms of apoptosis induced by β-elemene. Methods Human esophageal carcinoma cell EC9706 were incubated in vitro. Cells were treated with different concentrations of β-elemene（ 0、10、20、30、40、50 μg / ml） for 24 h or treated with 50 μg / ml β-elemene for 0,6,12 and 24 h,and the proliferation of EC9706 cells was evaluated by CCK-8. EC9706 cells were treated with 50 μg / ml β-elemene for 24 h. The apoptosis were tested by flow cytometry and the activity of caspase-3 was measured by spectrophotometry. Results The proliferation of EC9706 cells was inhibited in the β-elemene in a certain range of concerntration（ 0 - 50 μg / ml）. With the increase of β-elemene concerntration,the proliferation of EC9706 cells was inhibited in a concerntration-dependent manner（ P〈 0. 05）. Compared with EC9706 cells treated in 0 hours,the proliferation of EC9706 cells was significantly decreased in 6,12 and 24 hours（ P〈 0. 05）. The apoptosis rate was greater when EC9706 cells were incubated in 50 μg / ml β-elemene for 24 h than that in 0 μg / ml β-elemene（ P〈 0. 05）. At the meantime,the activity of caspase-3 in EC9706 cells was upregulated by β-elemene. Conclusion The proliferation of EC9706 cells is inhibited by β-elemene in the concerntration-dependent and time-dependent manner. β-elemene induced apoptosis of EC9706 cells,which might be due to the activation of caspase-3.

关 键 词：Β-榄香烯 人食管癌细胞 增殖 凋亡 

分 类 号：R735.1[医药卫生—肿瘤]