过量氟对大鼠切牙细胞凋亡及Fas表达影响的研究  

Effects of excessive fluoride on cell apoptosis and fas expression in rat incisor cells

作　　者：贺凌飞[1] 谢谦[1] 周小燕[1] 康成容[1] 王玉栋[1] 邹志辉[2] 余日安[2] 

机构地区：[1]广东药学院附属第一医院口腔科,广东广州510080 [2]广东药学院公共卫生学院劳动卫生与环境卫生学系,广东广州510310

出　　处：《临床口腔医学杂志》2016年第1期30-32,共3页Journal of Clinical Stomatology

基　　金：广东省医学科研基金资助项目(A2014365)

摘　　要：目的:建立饮水型氟斑牙大鼠模型,观察氟对大鼠切牙细胞凋亡和Fas表达的影响。方法:20只SPF级雄性SD大鼠随机分为对照组,低、中、高氟组,分别自由饮用浓度为0、10 mg/L、50 mg/L、100 mg/L的氟化钠水溶液120 d,采用硫代巴比妥酸法、流式细胞和免疫组化技术分别检测各组大鼠切牙细胞中丙二醛的含量、细胞凋亡率及Fas死亡受体表达水平。结果:雄性SD大鼠饮用高氟水120 d后,其切牙呈现典型的氟斑牙改变。大鼠切牙细胞MDA含量和细胞凋亡较对照组没有显著升高,但均呈上升趋势,且氟诱导切牙细胞凋亡与染氟剂量呈剂量-效应关系(r=0.9240);各染氟剂量组与对照组比较,大鼠切牙细胞Fas表达明显升高,高氟组大鼠切牙细胞中Fas表达较低、中氟组Fas表达明显升高(P>0.05)。大鼠切牙细胞的Fas表达水平与凋亡率之间存在显著相关关系(r=0.9375,P<0.01)。结论:过量氟可以激活切牙细胞内Fas凋亡通路,Fas信号通路介导的切牙细胞凋亡可能是氟斑牙发生的主要机制之一。Objective：To establish rat model for dental fluorosis through drinking water and study the effect of fluoride on cell apoptosis and Fas expression in rat incisor cells of male SD rats and explore the molecular pathogenesis of dental fluorosis. Method：20 male SD rats were divided into 4 groups randomly and provided with distilled water containing Na F at the doses of 0,10,50 and 100 mg/L for 120 d respectively.MDA content and cell apoptosis in rat incisor cells were measured by TBA method and flow cytometry（FCM）,respectively. Fas expression was measured with immunohistochemistry.Result：After 120d＇s fluoride exposure,Na F at the doses of 10,50 and 100 mg/L could cause dental fluorosis at rat mandibular incisor obviously. Compared with control groups,MDA content and cell apoptosis of rat incisor cells in all fluoride groups did not increased obviously. Rat incisor cell apoptosis induced by fluoride increased in a dose-dependent manner and relative coefficient was 0.9240. Compared with control groups,Fas expression in all fluoride groups increased obviously and Fas expression in high fluoride group was higher obviously than those of low and middle fluoride groups（P〈0.05）. The apoptosis rate displayed a close relationship with Fas expression in incisor cells of rats（r =0.9375,P〉0.01）.Conclusion：Excessive fluoride could activate Fas apoptosis pathway in rat incisor cells to stimulate cell apoptosis,which maybe one of important development mechanisms of dental fluorosis.

关 键 词：氟 切牙细胞 细胞凋亡 FAS 

分 类 号：Q786[生物学—分子生物学] R780.2[医药卫生—口腔医学]